Effect of Genetic polymorphisms of the Tumor Suppressor gene TP53 pathway in Saudi smokers and non-smokers
Background: Tumor suppresser gene (TP53) and its downstream genes (P21 and MDM2) play crucial roles in the regulation of DNA damage checkpoint (G1/S) of the cell cycle. Genetic polymorphisms in those genes have been implicated in developing various smoking-related diseases (SRDs). The aim of this study was to examine the potential association between cigarette smoking (CS) and the single nucleotide polymorphisms )SNPs( located in TP53, P21, and MDM2 genes, situated in the exons regions, among smokers and non-smoker from Saudi Arabian population. Methods: TP53 rs1042522 (C/G), P21 rs1801270 (A/C), and MDM2 rs769412 (A/G) were investigated by genotyping 568 blood specimens, 283 were obtained from male and female smokers, and 285 were obtained from male and female non-smokers. Results: Genetic and allelic alterations were detected between the rs1042522 variant tested here and the smoking patients in terms of patient age, patient gender, duration of CS, daily rate of CS, and types of smoking among Saudi smokers as compared to the control individuals. P21 rs1801270 polymorphism, however, was associated with allelic differences in smokers whose ages fall under 29 years, below 7 years of CS consumption, and among smokers who smoke shisha. In addition, our results showed that no genetic and allelic variations were detected between the MDM2 SNP rs769412 and the smoking subjects in all clinical parameters mentioned previously. Conclusion: Our results demonstrate that rs1042522 polymorphism has a higher effector role in all clinical parameters of the smoking population, which increases the potential risk of developing SRDs. Therefore, this polymorphism could be used as a novel diagnostic biomarker for the early diagnosis of several diseases caused by CS in the Saudi population.
The tumor suppressor gene TP53 and its downstream genes P21 and MDM2 play crucial roles in combating DNA damage at the G1/S cell cycle checkpoint. Polymorphisms in these genes can lead to the…
Background: Tumor suppresser gene (TP53) and its downstream genes (P21 and MDM2) play crucial roles in the regulation of DNA damage checkpoint (G1/S) of the cell cycle. Genetic polymorphisms in…