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Binu Antony

Associate Professor

Group Lead, Chemical Ecology and Functional Genomics Research lab

كلية علوم الأغذية والزراعة
Department of Plant Protection, Building no. 68, First Floor, Office no. 26, Lab no. 40.
المنشورات
مقال فى مجلة
2022

Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast

Bioproduction of ZETA pheromone

BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trap-ping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia(Cadra)cautella(Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bio-production technology for high-value storage pest control.

نوع عمل المنشور
Project
اسم الناشر
Wiley
رقم المجلد
78
الصفحات
1048–1059
مزيد من المنشورات
publications

BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption…

بواسطة Bao-Jian Ding, Hong-Lei Wang, Mohammed Ali Al-Saleh, Christer Löfstedt and Binu Antony
2022
تم النشر فى:
Wiley