Effect of using mannan-oligosaccharides and β-glucans as feed additives on growth, biochemical parameters, and immunity of Salmonella enterica serovar Enteritidis-challenged turkey

مقال فى مجلة

2026

تم النشر فى: https://doi.org/10.1007/s11259-026-11441-8

Turkey · Mannan-oligosaccharides · β-glucan · Salmonella enterica serovar Enteritidis · Performanc mmunity

This study aimed to detect the prevalence, antimicrobial susceptibility, and molecular characterization of some virulence
determinants in Salmonella recovered from 100 diseased turkeys in Dakahlia Governorate, Egypt. Twelve Salmonella
isolates were recovered from the diseased turkeys with an overall prevalence of 12%. The recovered isolates were serotyped
into 4 serovars: (S. enterica serovar Enteritidis, S. enterica serovar Typhimurium, S. enterica serovar Infantis
and S. enterica serovar Heidelberg). Additionally, a 21-d trial investigated the effects of dietary Mannan-oligosaccharides
(MOS) and β-glucans as feed additives on growth performance parameters, intestinal morphology, and blood biochemical,
immunological parameters, and fecal shedding of S. enterica serovar Enteritidis-challenged turkey. A total of 300 Oneday-
old turkey poults were allocated into five treatment groups (100 poults for each) in a completely randomized design:
group 1: a negative control group (non-challenged, non-supplemented), group 2: a positive control group (S. enterica
serovar Enteritidis-challenged at 7th day, non-supplemented), group 3 and 4: two challenged groups supplemented with
a mixture of MOS and β-glucan at 1 & 2 g/kg diet, respectively, and T5: a challenged group and treated with Florfenicol
antibiotic on the 8th day of the experiment at a dose of 1 ml /liter drinking water for five consecutive days. The in
vivo experimental results showed that birds in the positive control group showed reduced growth performance compared
with the negative control group. Supplementation with MOS and β-glucan, particularly at 2 g/kg ration, significantly
improved body weight gain, and feed conversion ratio, in addition to reduced clinical signs and mortality compared with
the positive control group. Overall, MOS and β-glucan at 2 g/kg restored growth performance close to negative control
and showed better feed efficiency than the antibiotic-treated group. MOS and β-glucan supplementation, particularly at
2 g/kg, significantly increased globulin, thyroid hormones, and immunoglobulins (IgA, IgG, and IgM), and IL1β, and
interferon-gamma (IFN-γ) compared with the control groups. Histopathological examination revealed normal liver and
spleen structures in the negative control group, while the positive control group showed marked degenerative and inflammatory
changes in the liver and lymphoid depletion in the spleen. Supplementation with MOS and β-glucan, particularly
at 2 g/kg, markedly improved hepatic and splenic histological structures with mild inflammatory changes, approaching
the normal architecture. The antibiotic-treated group also exhibited nearly normal histological features. Bacteriological
examinations revealed lower Salmonella shedding in the MOS and β-glucans 2 g/kg group in comparison with the positive
control and the MOS and β-glucans 1 g/kg groups. In conclusion, dietary addition of MOS and β-glucans 2 g/kg in
turkey diets improved growth performance, immune status, and hepatic and splenic histological structures in addition to
lowering Salmonella fecal shedding.

• نوع عمل المنشور: original article
• اسم الناشر: Springers
• مدينة النشر: Riyadh
• رقم المجلد: 50
• رقم الانشاء: 516
• مجلة/صحيفة: Veterinary Research Communications
• الصفحات: 1-17