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د. عبدالله بن عبدالعزيز العمر

Assistant Professor

أستاذ نواقل الأمراض الوبائية المساعد

كلية علوم الأغذية والزراعة
68G21
المنشورات
مقال فى مجلة
2024

Rapid detection of West Nile and Dengue viruses from mosquito saliva by loop-mediated isothermal amplification and displaced probes

Arthropod-borne viruses are major causes of human and animal disease, especially in endemic low- and middle-income countries. Mosquito-borne pathogen surveillance is essential for risk assessment and vector control responses. Sentinel chicken serosurveillance (antibody testing) and mosquito pool screening (by RT-qPCR or virus isolation) are currently used to monitor arbovirus transmission, however substantial time lags of seroconversion and/or laborious mosquito identification and RNA extraction steps sacrifice their early warning value. As a consequence, timely vector control responses are compromised. Here, we report on development of a rapid arbovirus detection system whereby adding sucrose to reagents of loop-mediated isothermal amplification with displaced probes (DP-LAMP) elicits infectious mosquitoes to feed directly upon the reagent mix and expectorate viruses into the reagents during feeding. We demonstrate that RNA from pathogenic arboviruses (West Nile and Dengue viruses) transmitted in the infectious mosquito saliva was detectable rapidly (within 45 minutes) without RNA extraction. Sucrose stabilized viral RNA at field temperatures for at least 48 hours, important for transition of this system to practical use. After thermal treatment, the DP-LAMP could be reliably visualized by a simple optical image sensor to distinguish between positive and negative samples based on fluorescence intensity. Field application of this technology could fundamentally change conventional arbovirus surveillance methods by eliminating laborious RNA extraction steps, permitting arbovirus monitoring from additional sites, and substantially reducing time needed to detect circulating pathogens.

نوع عمل المنشور
بحث علمي
اسم الناشر
PLoS ONE 19(2):e0298805
مزيد من المنشورات
publications

This article provides an overview of Rift Valley fever virus (RVFV) history, geographic distribution, vectors, hosts, epidemiology, and control, and is intended to deliver important information…

بواسطة Abdullah A Alomar, Lindsay P Campbell, Derrick K Mathias, and Nathan D Burkett-Cadena
2023
تم النشر فى:
EDIS ENY-2099
publications

Arthropod-borne viruses are major causes of human and animal disease, especially in endemic low- and middle-income countries. Mosquito-borne pathogen surveillance is essential for risk assessment…

بواسطة Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
2024
تم النشر فى:
PLoS ONE 19(2):e0298805
publications

This article outlines the use of the bacterium Wolbachia pipientis as a tool to control mosquito populations and reduce the spread of mosquito-borne diseases

بواسطة Abdullah A Alomar, Leena Salam, Daniel W Pérez-Ramos, and Eric P Caragata
2024
تم النشر فى:
EDIS