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نواف بن مساعد العتيبي Nawaf Musaid Alotaibi

Demonstrator

عضو هيئة تدريس Demonstrator, College of Medicine, Faculty of Internal Medicine, Pulmonology Unit

كلية الطب
مبنى كلية الطب، الدور الثاني، قسم الباطنة

Sputum Microbiome is Associated with 1-Year Mortality Following COPD Hospitalizations.

Filho, Fernando Sergio Leitao . 2018

COPD Sputum microbiome Mortality

Rationale: Lung dysbiosis promotes airway inflammation and decreased local immunity, potentially playing a role in the pathogenesis of acute exacerbations of COPD (AECOPD). We determined the relationship between sputum microbiome at
the time of AECOPD hospitalization and 1-year mortality in a COPD cohort.
 

Methods: We used sputum samples from 102 patients hospitalized due to AECOPD. All subjects were followed for one year after discharge. The microbiome profile was assessed through sequencing of 16S rRNA gene. Microbiome analyses were performed according to 1-year mortality status. To investigate the effect of alpha diversity measures and taxa features on time to death, we applied Cox proportional-hazards regression models and obtained hazard ratios (HR) associated with  these variables
 

Measurements and Main Results: We observed significantly lower values of alpha diversity (Richness, Shannon Index, Evenness, and Faith’s Phylogenetic Diversity) among non-survivors (n=19, 18.6%) compared to survivors (n=83, 81.4%). Beta diversity analysis also demonstrated significant differences between both groups (adj. PERMANOVA, p=0.010). The survivors had a higher relative abundance of Veillonella; in contrast, non-survivors had a higher abundance of Staphylococcus. The adjusted HRs for 1-year mortality increased significantly with decreasing alpha diversity. We also observed lower survival among patients in whom sputum samples were negative for Veillonella (HR: 13.5, 95% CI: 4.2 –
43.9, p<0.001) or positive for Staphylococcus (HR: 7.3, 95% CI: 1.6 – 33.2, p=0.01). 
 

Conclusion: The microbiome profile of sputum in AECOPD is associated with 1-year mortality and may be used to identify subjects with a poor prognosis at the time of hospitalization.

 

Magazine \ Newspaper
American Journal of Respiratory and Critical Care Medicine
more of publication
publications

Background: Etiologies of acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are heterogeneous. We phenotyped severe AECOPD based on molecular pathogen…

by Nawaf M Alotaibi, Virginia Chen, Zsuzsanna Hollander, Jonathon A Leipsic , Cameron J Hague, Darra T Murphy, Mari L DeMarco, J. Mark FitzGerald, Bruce M McManus, Raymond T Ng, Don D Sin
2019
publications

Rationale: Lung dysbiosis promotes airway inflammation and decreased local immunity, potentially playing a role in the pathogenesis of acute exacerbations of COPD (AECOPD). We determined the…

by Fernando Sergio Leitao Filho, Nawaf M Alotaibi, David Ngan, Sheena Tam , Julia Yang, Zsuzsanna Hollander, Virginia Chen, J. Mark FitzGerald, Corey Nislow, Janice M leung, SF Paul Man, Don D Sin
2018
publications

Introduction: The use of beta-blockers in chronic obstructive pulmonary disease (COPD) is controversial, primarily due to concerns that they may worsen lung function and attenuate bronchodilator…

by Nawaf M Alotaibi, Fernando Sergio Leitao Filho, Kei Yamasaki, David A Ngan, Don D Sin
2018