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محمد بن عبدالرحمن السنيدي

Associate Professor

رئيس قسم الصيدلانيات

كلية الصيدلة
قسم الصيدلانيات, كلية الصيدلة, مبنى رقم 23 ,الدور الأرضي , مكتب أأ 101
publication
Journal Article
2014

Protein comparability assessments and potential applicability of high throughput biophysical methods and data visualization tools to compare physical stability profiles

Volkin, Mohammad A. Alsenaidy, Nishant K. Jain, Jae H. Kim, C. Russell Middaugh and David B. . 2014

In this review, some of the challenges and opportunities encountered during protein comparability assessments are summarized with an emphasis on developing new analytical approaches to better monitor higher-order protein structures. Several case studies are presented using high throughput biophysical methods to collect protein physical stability data as function of temperature, agitation, ionic strength and/or solution pH. These large data sets were then used to construct empirical phase diagrams (EPDs), radar charts, and comparative signature diagrams (CSDs) for data visualization and structural comparisons between the different proteins. Protein samples with different sizes, post-translational modifications, and inherent stability are presented: acidic fibroblast growth factor (FGF-1) mutants, different glycoforms of an IgG1 mAb prepared by deglycosylation, as well as comparisons of different formulations of an IgG1 mAb and granulocyte colony stimulating factor (GCSF). Using this approach, differences in structural integrity and conformational stability profiles were detected under stress conditions that could not be resolved by using the same techniques under ambient conditions (i.e., no stress). Thus, an evaluation of conformational stability differences may serve as an effective surrogate to monitor differences in higher-order structure between protein samples. These case studies are discussed in the context of potential utility in protein comparability studies.

Publication Work Type
بحث علمي محكم
Magazine \ Newspaper
Frontiers in Pharmacology
more of publication
publications

The structural integrity and conformational stability of various IgG1-Fc proteins produced from the yeast Pichia pastoris with different glycosylation site occupancy (di-, mono-, and…

2014
publications

In this review, some of the challenges and opportunities encountered during protein comparability assessments are summarized with an emphasis on developing new analytical approaches to better…

by Mohammad A. Alsenaidy, Nishant K. Jain, Jae H. Kim, C. Russell Middaugh and David B. Volkin
2014
publications

Acidic fibroblast growth factor (FGF-1) is an angiogenic protein which requires binding to a polyanion such as heparin for its mitogenic activity and physicochemical stability.

by محمد السنيدي
2011