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Haneen Abdullah ALMazroua- حنين عبدالله المزروع

Assistant Professor

Faculty

كلية الصيدلة
Building 8, 1st floor, office no. 14
publication
Journal Article
2019

Assessment of DNA repair efficiency in the inbred BTBR T+tf/J autism spectrum disorder mouse model exposed to gamma rays and treated with JNJ7777120

H.A, Al-Mazroua H.A., Alomar H.A., Ahmad S.F., Attia M.S., Nadeem A., Bakheet S.A., Alsaad A.M.S., Alotaibi M.R., . 2019

Information regarding DNA repair in autism is limited to a few studies, which have reported inconsistent results. Therefore, we designed a study to determine whether DNA repair efficiency is altered in autism and to investigate whether the H4 ligand JNJ7777120 can enhance DNA repair efficiency in BTBR T+tf/J (BTBR) mice; we also attempted to elucidate the mechanism(s) underlying this amelioration. Evaluation of DNA damage using the comet assay on bone marrow cells showed increased levels of DNA damage in BTBR mice compared with agematched control C57BL/6J mice. Conversely, BTBR animals pretreated with 20 mg/kg JNJ7777120 for five days exhibited significant decreases in DNA damage compared with that of control BTBR mice. Our results also indicated higher sensitivity of BTBR mice exposed to gamma rays to DNA damage generation. A marked difference was observed between BTBR and C57BL/6J mice at different sampling times after irradiation, with BTBR mice showing a higher percentage of DNA damage and slower repair rate than that of C57BL/6J mice. JNJ7777120 led to enhanced repair of the DNA damage induced by radiation when administered to BTBR mice five days prior to radiation. Additionally, oxidative stress in BTBR mice was significantly elevated with a reduced GSH/GSSG ratio; significant amelioration was subsequently observed in JNJ7777120-pretreated BTBR mice.
Furthermore, repetitive behaviors were also attenuated in BTBR mice by JNJ7777120 treatment without altering locomotor activity. Our results suggest that JNJ7777120 can be developed for use as a therapeutic agent to enhance DNA repair efficiency in autism spectrum disorder.

Publication Work Type
PHD
Volume Number
93
Magazine \ Newspaper
Progress in Neuropsychopharmacology & Biological Psychiatry
Pages
189–196
more of publication
publications

Autism spectrum disorder (ASD) is a prevalent neurodevelopmental disorder characterized by deficits in social interaction, communication, and repetitive behaviors. BTBR T+ Itpr3tf/J (BTBR) mice, a…

by Sheikh F. Ahmad, Mushtaq A. Ansari, Ahmed Nadeem, Saleh A. Bakheet, Moureq R. Alotaibi, Abdullah F. Alasmari, Musaad A. Alshammari, Haneen A. Al-Mazroua, Sabry M. Attia
2019
publications

Autism is a neurodevelopmental disorder characterized by deficits and qualitative impairments in communication and implicit skill learning. Its prevalence is higher than previous estimates, and…

by Sheikh F. Ahmad, Ahmed Nadeem, Mushtaq A. Ansari, Saleh A. Bakheet, Haneen A. Al-Mazroua, Mohammad R. Khan, Abdullah F. Alasmari, Wael A. Alanazia, Homood M. As Sobeaia, Sabry M. Attia
2019
publications

Information regarding DNA repair in autism is limited to a few studies, which have reported inconsistent results.

by Al-Mazroua H.A., Alomar H.A., Ahmad S.F., Attia M.S., Nadeem A., Bakheet S.A., Alsaad A.M.S., Alotaibi M.R., H.A
2019