Skip to main content
User Image

Dr. Amr T. M. Saeb

Assistant Professor

Head of Genetics and Microbiology

كلية الطب
Strategic Center for Diabetes Research, College of Medicine - King Saud University
publication
Journal Article
2017

The use of next-generation sequencing in the identification of a fastidious pathogen: a lesson from a clinical setup

Tayeb, Amr TM Saeb, Mohamed Abouelhoda, Manojkumar Selvaraju, Sahar I Althawadi, Maysoon Mutabagani, Mohammad Adil, Abdullah Al Hokail, Hamsa T . 2017

Clostridium haemolyticum C. novyi sensu lato next-generation sequencing techniques bioinformatics

Clostridium haemolyticum is the causal agent of bacillary hemoglobinuria in cattle, goat, sheep, and ruminants. In this study, we report the first recorded human-infecting C. haemolyticum strain collected from an 18-year-old woman diagnosed with acute lymphoblastic leukemia. After failure of traditional techniques, only next-generation sequencing (NGS) technology in combination with bioinformatics, phylogenetic, and pathogenomics analyses revealed that our King Faisal Specialist Hospital and Research Center (KFSHRC) bacterial isolate belongs to C. haemolyticum species. KFSHRC isolate is composed of 1 chromosome and 4 plasmids. The total genome size is estimated to be 2.7 Mbp with a low GC content of 28.02%. Comparative pathogenomics analysis showed that C. haemolyticum KFSHRC isolate is a potential virulent pathogenic bacterium as it possesses the virulence factors necessary to establish an infection, acquire essential nutrients, resist antimicrobial agents, and tolerate hostile conditions both in the human host and in its surrounding environment. These factors are included in the main chromosome in addition to novel recombination of the plasmids, and they could be the reason for the incidence of that human infection. This work demonstrated the importance of using NGS in medical microbiology for pathogen identification. It also demonstrates the importance of sequencing more microbial samples and sharing this information in public databases to facilitate the identification of pathogenic microbes with better accuracy.

Publication Work Type
Original Research
Volume Number
13
more of publication
publications

Clostridium haemolyticum is the causal agent of bacillary hemoglobinuria in cattle, goat, sheep, and ruminants. In this study, we report the first recorded human-infecting C.…

by Amr TM Saeb, Mohamed Abouelhoda, Manojkumar Selvaraju, Sahar I Althawadi, Maysoon Mutabagani, Mohammad Adil, Abdullah Al Hokail, Hamsa T Tayeb
2017
publications

Background

by Amr T. M. Saeb, Khalid A. Al-Rubeaan, Mohamed Abouelhoda, Manojkumar Selvaraju & Hamsa T. Tayeb
2017
publications

Background: A reciprocal relationship between oral health and systemic disease, such as type 2 diabetes, has
been suggested, whereby a systemic disease is a predisposing factor for oral…

by Amr T.M. Saeb∗, Khalid A. Al-Rubeaan, Khalid Aldosary, G.K. Udaya Rajac, Balavenkatesh Manic, Mohamed Abouelhodad, Hamsa T. Tayeb
2019