In vitro antimicrobial activity of some medicinal plants found in Saudi Arabia

Journal Article
Perveen, Kahkashan . 2014
Publication Work Type: 
بحث منشور
Magazine \ Newspaper: 
Journal of Pure and Applied Microbiology ISSN 0973-7510
Volume Number: 
(Vol. 8 (3
p. 2129-2134
Publication Abstract: 

he methanolic extracts of

Citrullus colocynthis, Rhazya





Zygophyllum coccineum

were evaluated for the antimicrobial activity


Pseudomonas aeruginosa

ATCC 27853,

Escherichia coli

ATCC 25922,

Staphylococcus aureus

ATCC 25928,

Streptococcus pyogenes

grp A (clinical isolate),

Bacillus subtilis

ATCC 6633 and

Candida albicans

ATCC 10231


The extract of different

plants tested showed varying degree of inhibitory activity against the tested pathogens.

Extract of

C. colocynthis

showed the highest activity followed by

R. stricta,




exhibited least activity. The gram positive bacteria were observed to be more

sensitive to plant extracts as compare to gram negative bacteria. Moreover, all extract

were effective against

C. albicans

. While, only the extract of

C. colocynthis

was able to

inhibit the growth of

P. aeruginosa

(18.3 mm). The maximum zone of inhibition was

observed against

S. aureus

(31.3mm) by the extract of

C. colocynthis

and the MIC against

the same bacteria was 0.2 mg/ml. The GCMS analysis of

C. colocynthis

extract showed the

presence of several esters, glycosides, alkaloids and flavonoids. However, the main

component detected in the extract was l-(+)-Ascorbic acid 2,6-dihexadecanoate; a known

hyaluronidase inhibitor. Therefore, the results showed that

C. colocynthis

possesses a

broad spectrum of activity against pathogenic bacteria responsible for the most common

microbial diseases

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