Cytotoxicity and Genotoxicity of Copper Oxide Nanoparticles in Human Skin Keratinocytes Cells
The wide scale use of copper oxide nanoparticles (CuONPs) due to their unique properties and important applications in magnetic,
thermal, electrical, sensor devices, and cosmetics makes human beings more prone to the exposure of CuONPs and its potential
adverse effects. Exposure to such nanoparticles is mainly through skin and inhalation. Therefore, the aim of the present study was to
assess the apoptotic and genotoxic potential of CuONPs (50 nm) in the human skin epidermal (HaCaT) cells and its underlying
mechanism of cellular toxicity. Significant decreases in cell viability were observed with CuONPs exposure in a dose- and timedependent manner and also induced significant reduction in glutathione and induction in lipid peroxidation, catalase, and superoxide
dismutase in HaCaT cells. A significant increase in caspase-3 activity was observed with CuONPs exposure in HaCaT cells indicating
apoptosis. Apoptosis or necrosis was confirmed with fluorescent staining (acridine orange and propidium iodide). The CuONPs also
induced DNA damage that was mediated by oxidative stress. This study investigating the effects of CuONPs in human skin cells has
provided valuable insights into the mechanism of potential toxicity induced by CuONPs.
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Objective
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