Cytotoxic assessment of liver cancer cells (HepG2) with raw, functionalized multiwalled carbon nanotubes and their comparison with nanohydroxyapatite
The carbon based nanostructured material exhibit unique and versatile properties and due this reason its largely applied in different fields. The present work was designed for to know the cytotoxic and genetic responses against liver cancer cells and was compared with the artificial bone implanted material hydroxyapatite (HA). The raw multiwalled carbon nanotubes (RMWCNTs) surfaces were turns to functionalized carbon nanotubes (FMWCNTs) via acid digestion process. The surface functional groups were identified through fourier transform infra red spectroscopy (FTIR) instruments, while structural detail of RMWCNTs, FMWCNTs and HA powder were observed via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) consequently. The RMWCNTs, FMWCNTs and HAs powder were utilized against liver cancer (HepG2) cells to evaluate the rate of effectiveness against cells. The viability of grown cells were examined through MTT assay with the impact of doses of CNTs and HA powder (1 μg/mL, 2 μg/mL, 5 μg/mL,10 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL). The cells morphology was verified via inverted microscopy at different doses of CNTs and HA with control. Beside this, gene expression study was also performed for to know the apoptosis caused with CNTs and HA. The study was scrutinized with apoptotic and anti-apoptotic marker genes (p53, Bax, Caspase 3 and Bcl2) through quantitative polymerase chain reaction (qPCR). The qPCR data reveals that the up-regulation in gene expression and it shows that the CNTs, HAs are responsible for cells death with treatment at 50 μg/mL in 24 h incubation. The possible discussion related to cells death caused with CNTs and HAs were also described.
Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…
Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…
Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…