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Dr. Javed Ahmad

Assistant Professor

Assistant Professor

كلية العلوم
Room # 2B 121, Second Floor, Bld # 5, Department of Zoology, College of Science
publication
Journal Article
2022

Strontium-Doped Nickel Oxide Nanoparticles: Synthesis, Characterization, and Cytotoxicity Study in Human Lung Cancer A549 Cells

In this manuscript, the grown and annealed strontium-doped nickel oxide nanoparticles (SrNiONPs) were synthesized using a precipitation method with nickel nitrate and strontium nitrate as precursor agents with trisodium citrate. Various characterization techniques, including X-ray diffraction pattern (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), UV-visible, and zeta sizer, were used to thoroughly examine the samples. The XRD pattern (21 nm) was used to calculate the size, phases, and crystallinity of the material (SrNiONPs). In addition to characterization, the material was tested for cytotoxicity in lung cancer cells (A549). The viability test in A549 cells was performed using [3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide] (MTT) and Neutral Red Uptake (NRU) assay with SrNiONPs concentration ranging from 1 to 100 μg/mL. According to the MTT and NRU data, the toxicity studies are dose-dependent. SrNiONPs also increased reactive oxygen species (ROS) and were involved in apoptosis (A549 cells). Furthermore, quantitative PCR (qPCR) data revealed that the mRNA levels of apoptotic genes marker like p53, bax, and caspase-3 were upregulated, whereas bcl-2, an anti-apoptotic gene, was downregulated. As a result, apoptosis was mediated by the p53, bax, caspase3, and bcl-2 pathways, implying a potential mechanism by which SrNiONPs mediate their toxicity.

Publisher Name
Springer
Volume Number
200
Magazine \ Newspaper
Biological Trace Element Research
Pages
1598-1607
more of publication
publications

Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…

by Maqsood A. Siddiqui , Rizwan Wahab , Quaiser Saquib , Javed Ahmad , Nida N. Farshori , Ebtesam S. Al-Sheddi , Mai M. Al-Oqail , Shaza M. Al-Massarani , Abdulaziz A. Al-Khedhairy.
Published in:
ELZEVIER
publications

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…

2023
Published in:
ELZEVIER
publications

Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…

by Javed Ahmad a, Rizwan Wahab, Maqsood A. Siddiqui, Quaiser Saquib, Abdulaziz A. Al-Khedhairy.
2023
Published in:
ELZEVIER