Hazards of low dose flame-retardants (BDE-47 and BDE-32): Influence on transcriptome regulation and cell death in human liver cells
Al-Arifi, Quaiser Saquib · Maqsood A. Siddiqui · Javed Ahmed · Abdullah Al-Salim · Sabiha M. Ansari · Mohammad Faisal · Abdulaziz A. Al-Khedhairy · Javed Musarrat · Hend A. AlWathnani · Abdulrahman A. Alatar · Saud A . 2016
Abstract
We have evaluated the in vitro low dose hepatotoxic effects oftwo flame-retardants (BDE-47 and BDE-32) in HepG2 cells. Both congeners declined the viability of cells in MTT and NRU cell viability assays. Higher level of intracellular reactive oxygen species (ROS) and dysfunction of mitochondrial membrane potential (m) were observed in the treated cells. Comet assay data confirmed the DNA damaging potential of both congeners. BDE-47 exposure results in the appearance of subG1 apoptotic peak (30.1%) at 100 nM, whileBDE-32 arrestedthe cells in G2/Mphase.Among the set of 84 genes,BDE-47 inducesdownregulation of majority of mRNA transcripts, whilst BDE-32 showed differential expression of transcripts in HepG2. The ultrastructural analysis revealed mitochondrial swelling and degeneration of cristae in BDE-47 and BDE-32 treated cells. Overall our data demonstrated the hepatotoxic potential of both congeners via alteration of vital cellular pathways.
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