Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson,

Journal Article

2022

Published in: https://doi.org/10.1016/j.jksus.2022.102265.

abstract
The current research work was planned to investigate genotoxicity in fish to screen and monitor the
aquatic ecosystem. Epinephalus chlorostigma (Hamour) was collected from the contaminated areas of
‘‘Tarut Island” (26.571N 50.056E) in the Arabian Gulf near Dammam, Saudi Arabia. DNA fragmentation
was detected by Comet assay and Micronucleus assay. Heavy metals’ water quality parameters and concentrations (Pb, Cr, Zn, Mn, Cu, Cd, Sn, and Hg) were extensively higher than the WHO permissible limits.
They were more than enough to have adverse effects on fish health. The highest DNA fragmentation was
observed in E. chlorostigma, indicating its most heightened sensitivity to pollution. E. chlorostigma showed
comet head diameters 63.33 ± 2.2, 83.59 ± 3.38, and 66.28 ± 2.13px from S1-S3, respectively. E. chlorostigma erythrocytes showed comet tail lengths as 16.66 ± 1.65, 16.20 ± 1.63, and 19.07 ± 1.81px from S1-
S3. DNA damage was found to be 19.14 ± 1.38, 16.38 ± 1.26, and 19.95 ± 1.33 % from S1-S3, respectively.
The tail moment was observed as 6.46 ± 0.79, 4.72 ± 0.69, and 7.14 ± 1.08, while the olive moment was
recorded as 5.31 ± 0.51, 5.14 ± 0.52, and 6.01 ± 0.49, respectively from S1-S3. The highest frequency for
single micronucleus induction, double micronucleus induction, and nuclear abnormalities was observed
in E. chlorostigma collected from the polluted site of the study area. This study proposes that this fish
species and novel DNA damage assays could be the best tools for toxicity screening and monitoring water
bodies

• Publisher Name: ELSEVIER
• Publishing City: NETHERLANDS
• Volume Number: 34
• Issue Number: 7
• Magazine \ Newspaper: Journal of King Saud University - Science,
• Pages: 102265