Laboratory Skills (MBI- 240)

 

Course Specialization

The basic techniques in Microbiology laboratories (Microbiology Lab. Organization and management The microcopy technique, Kinds of microscopes, Sterilization techniques – Pure culture techniques – Morphology , growth and staining technique – Effect of physical and chemical factors on microorganisms physiological activities. -Antibiotic production- microbial Enzymatic activities Medical microbiology.

The following are some of the techniques covered in this course.

• General precautions and safety rules
• Sterilization and Disinfection skills
• Microscopy.
• Preparation of microbial cultivation media
• Isolation and purification of microorganisms from soil
• Preparation of microbial slides, Gram staining
• Isolation and purification of fungi from different plant parts
• Fungal staining and microscopic techniques (Fungi: Rhizopus, Penicillium)
• Pure culture techniques
• Physical and chemical factors affecting growth and activities of microorganisms
• Microbial Enzyme activities
• Antibiotics susceptibility tests
• Isolation of microorganisms from the Human body (Throat and skin culture)