Endotoxemia, vitamin D and premature biological ageing in Arab adults with different metabolic states

مقال فى مجلة
نوع عمل المنشور
Masters
رابط النشر على الانترنت
ملخص المنشورات

There are limited studies on the association of endotoxin, a potent mediator of gut-derived inflammation
and telomere length (TL). We investigated (1) the influence of adiposity on endotoxin and TL amongst
Saudi adults according to type 2 diabetes mellitus (T2DM) status and (2) the influence vitamin D may
have on TL attrition. Anthropometric data and fasting blood samples were taken from 775 Saudi adults
visiting different primary care centers in Riyadh [387 T2DM and 388 non-T2DM]. TL, derived from
peripheral blood mononuclear cells, was analyzed by Quantitative real-time polymerase chain reaction
and circulating endotoxin levels by Limulus Amebocyte Lysate assay. Subjects were stratified based on
obesity and T2DM status. A significant lower TL was observed in the non-obese T2DM group as compared
with their non-obese, non-T2DM counterparts (p = 0.002). Significant inverse associations between TL,
endotoxin and endotoxin activity were observed in the cohort with obesity. Regression analysis showed
that endotoxin was a significant predictor for TL in all subjects and even after stratification according to
subgroups; with variances perceived in circulating TL stronger among non-T2DM obese (10%; p = 0.003)
than non-T2DM non-obese (12%; p = 0.007). Also, in the non-T2DM group, TL and HDL-cholesterol predicted
29% of the variances perceived in 25(OH)D (p < 0.001). Taken together these findings show that
circulating endotoxin and 25(OH)D are associated with premature biological ageing influenced by adiposity
and metabolic state; suggesting future intervention studies to manipulate gut microbiome and
or vitamin D levels may offer ways to mitigate premature TL attrition.