Molecular and Physiological Behavior Variations of Artemesia Monosperma for Seasonal Temperature Divergence in the Middle Region, Saudi Arabia
, 41- M. M. Ibrahim, Najat A. Boukhari and Gehan A. El-Gaely . 2012
Artemisia monosperma were collected from three independent locations in Riyadh during the periods between June 2009 –Feb. 2010 . The goal of this study is to compare the physiological and biochemical strategies during temperature seasonal variation in Artemisia monosperma that is divergent in photosynthetic pigments and antioxidant system. Photosynthetic pigments, including chl a, b and carotenoid were significantly varied according to temperature divergence in different seasons. Tissue malondialdehyde (MDA) content which used as an oxidative stress indices was greatly enhanced by % in Artemisia monosperma collected during winter (at 9ºC) in comparison with plants collected during summer period(at 38ºC). Differences in accumulation of some antioxidant metabolites and enzymes activities during seasonal variations in temperature were investigated. Total ascorbate was markedly accumulated during winter period (especially at 9&15 ºC). Also antioxidant enzyme
activities were significantly enhanced in Artemisia monosperma during winter period compared with summer period. the different temperatures gave different results, which were quite significant POD P<0.05; PPO, P<0.05) in showing an inverse trend in relation to the concentration of total phenols Zymogram analysis showed the induction of two isozymes bands in A. monosperma subjected to high temperature 38 ºC and 47ºC.
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