550 كيح - البيولوجيا الجزيئية للجين

The goal of the course is to provide graduate students with a deeper understanding of the biochemistry of nucleic acids and the biochemical mechanisms that control the replication, maintenance, and expression of the genetic material of model organisms. 

 

• Course Description

The course provides a comprehensive coverage of the major topics in molecular biology including, review of genes and their structure, transcription, replication and translation in prokaryotic cell, eukaryotic cell and viruses. Cell differentiation and cell control of cell proliferation at the molecular level.

• Objectives

1. To provide a comprehensive understanding of eukaryotic genome, its structure, variations, functions, abnormalities and control of gene expression
2. To have an inclusive knowledge about the mechanisms damaging the genome, and the mechanisms that correct these defects in vivo and how these defects may lead to complex diseases such as cancer.
3. To acquire knowledge in epigenetic modification strategies.

 

Topics
Elements of DNA: genes, pseudo-genes transposable elements, repetitive DNA, etc. Eukaryotic and prokaryotic genome; Fundamental features of eukaryotic and prokaryotic genes 
The hallmarks of cancer- DNA damage sources.
Mutations- DNA repair mechanisms 
DNA repair defects in cancer developments
DNA repair pathways in cancer therapy and resistance 
Transcription: machinery and regulation (activators and repressors, histone modification, methylation). Epigenetic modification of the genome. Control of gene expression.  RNA interference as a mechanism for control of gene expression, splicing and connections with human diseases.
Translation: mechanisms, regulation, post-translational modifications
Bioinformatics.