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Mohammad Shamsul Ola

Professor

Faculty

كلية العلوم
Research Group of Diabetic Retinopathy and Biochemical Toxicology and Nanobiochemistry; 2A 61, PO Box 2455, Building 5, Department of Biochemistry, College of Science, King Saud University, Riyadh
المنشورات
مقال فى مجلة
2019

Molecular basis for increased lactate formation in the Müller glial cells of retina.

KF, Ola MS, LaNoue . 2019

Müller glial cells are highly metabolic active cells that compensate for the high energy demand of retinal neurons. It has been believed that glucose provides the energy needs by the complete oxidation within Müller cells. However, numerous studies indicated that glial cells convert the majority of glucose to lactate, which may serve as an energy source for neurons. It is still not well understood why within glia, glucose is not completely oxidized under aerobic glycolysis conditions. The aspartate glutamate carrier (AGC) is a major component of the malate-aspartate shuttle (MAS) responsible for transporting the reducing equivalent of glycolysis to the mitochondria for the complete oxidation of glucose. Here, we report the absence of AGC within Müller glial cells which impairs the ability to oxidize glucose. We investigated the expression and localization of AGC and its isoforms (aralar and citrin) in the intact rat retina. We also analyzed the expression and regulation of AGC and its metabolic activity within cultured Müller cells (TR-MUL). The results suggest that AGC and its isoforms seem to be neuronal, with no or low expression within Müller cells of the intact retina. The study of cultured Müller cells suggests a very low expression of AGC and a decreased metabolic activity of the carrier especially under cell differentiation conditions due to low serum and hydrocortisone treatments. Thus, these data give a molecular explanation of increased levels of lactate formation due to a lack of AGC in the retina by Müller glial cells.

نوع عمل المنشور
Research article
رقم المجلد
144
مجلة/صحيفة
Brain Research Bulletin
الصفحات
158-163
مزيد من المنشورات
publications

Background and Objectives: Phycocyanin, a water-soluble blue pigment extracted from cyanobacteria, is…

بواسطة Additiya Paramanya, Abeeb Oyesiji Abiodun, Mohammad Shamsul Ola, Ahmad Ali
2024
تم النشر فى:
Elsevier
publications

Ovarian cancer poses a significant challenge to women's health, necessitating innovative therapeutic approaches.

بواسطة Sabrina Lekmine, Ouided Benslama, Kenza Kadi, Antonio Ignacio Martín-García, Mohammad Shamsul Ola, Mustafa Abdullah Yilmaz, Ahmad Ali
2024
تم النشر فى:
Elsevier
publications

Objectives

بواسطة Md. Fahim Ahmad, Tariq Aziz, Nida Naseem, GG Hammad A. Shadab, Haseeb Ahsan, Hina Younus, Mohammad Shamsul Ola & Waseem A. Siddiqui
2024
تم النشر فى:
Springer