PHRM 213 Pharmaceutical analysis (1)

:Course Description

The course will include introduction to both volumetric and spectroscopic methods of analysis. The course will cover the basic principles of each method, mathematical treatment, laws, calculation, advantages, and limitations and finally, the applications of each method in the field of pharmaceutical analysis

 

List of topics 

Introduction to Pharmaceutical Analysis, Validation of Analytical Methods                          

Acid-Base Titrations                                                                        

Theories of acids and bases

pH of strong acids and bases, weak acids and bases and salts.                                    

Buffer solutions.

Titration curves

Acid-base indicators.

Acid-base titrations in non-aqueous medium

Precipitation Titrations                                                                                                     

Principles of precipitimetric titrations

Methods of precipitation titrations.   

Complex-formation Titrations                                                                                

 Principles of complexometric titrations

Titration curves of complexometric titrations           

Oxidation-Reduction Titrations                                                                               

Theories and definitions for of oxidation-reduction reactions

Nernst equation and oxidation potential of redox system

Redox titration curves.

Detection of end point in redox titrations     

Ultraviolet-visible spectrophotometry                                                                     

The electromagnetic spectrum

Laws of light absorption

Molecular absorption spectra

Instrumentation of spectrophotometry

Spectrophotometric     method            development   and      application       in quantitative analysis           

Fourier transforms infra-red spectroscopy                                                                      

Molecular emission spectrophotometry                                                                         

Theory of light emission

Fluorescence and phosphorescence and chemical structure

Instrumentation of fluorometry

Applications of fluorimetry in non-fluorescent compounds after their derivatization          

 Atomic absorption and emission spectrophotometry                                                                

Principles of atomic absorption and emission spectrophotometry

Instrumentation           of         atomic absorption       and      emission               spectrophotometry

Applications of atomic absorption and emission spectrophotometry

 

 

:Suggested Reading

Analytical Chemistry - Gary D. Christian, 7th edition

Quantitative Chemical Analysis – Daniel C. Harris 7th edition

Pharmaceutical Analysis – David G. Watson 4th edition

 

Module Questions

 
Short Answer

Analytical chemistry
The branch of chemistry that deal with the separation, identification and determination of components in a sample

Difference between qualitative and quantitative analysis
Qualitative / attempting to identify what materials are present in a sample
Quantitative/ determining how much of a material is present in a sample

Discuss and give example for using acid- base titration method for analysis
You should include in your answer

?What is acid-base titration

?Why does it use

?Endpoint and equivalence point