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Mohammad Nadeem

Associate Professor

Associate Professor

كلية العلوم
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, KSA
المنشورات
مقال فى مجلة
2018

SCAR Marker for Gender Identification in Date Palm (Phoenix dactylifera L.) at the Seedling Stage

Date palm (Phoenix dactylifera L.) is cultivated in arid and semiarid regions worldwide. Given the dioecious nature of this plant,
gender identification is very important at the seedling stage. Molecular markers are very effective tools that help in gender
identification at this stage. A sequence characterized amplified region (SCAR) marker linked to sex-specific regions in the
genome of date palm was developed. Of the 300 tested randomly amplified polymorphic DNA (RAPD) primers, only one
primer (OPC-06) produced reproducible band (294 bp) in male plants. The PCR product of this primer was cloned and
sequenced. The specific primers were synthesized for amplification of a 186 bp fragment in male date palm plants. These
primers were validated in male and female date palm plants, wherein the designed SCAR marker was reported only in male
plants and no amplification was observed in female plants. The developed SCAR marker was used with seedlings of date palm
and proved very effective in identification of gender.

اسم الناشر
International Journal of Genomics
مزيد من المنشورات
publications

Date palm (Phoenix dactylifera L.) is cultivated in arid and semiarid regions worldwide. Given the dioecious nature of this plant,
gender identification is very important at the seedling…

بواسطة Fahad Al-Qurainy,1 Abdulhafed A. Al-Ameri ,2 Salim Khan ,1 Mohammad Nadeem ,1 Abdel-Rhman Z. Gaafar ,1 and Mohamed Tarroum
2018
تم النشر فى:
International Journal of Genomics
publications

Maerua oblongifolia (Forssk.) A. Rich is an
important and rare medicinal plant from Saudi Arabia.
Natural regeneration of plant is very poor, occurs
by seed and tuber. Therefore…

بواسطة Fahad Al-Qurainy, Mohammad Nadeem*, Salim Khan, Saleh Alansi, Mohamed Tarroum
2018
تم النشر فى:
Fresenius Environmental Bulletin
publications

The plantlets of date palm were regenerated using somatic embryogenesis. The combination of phytohormones, 5 mg BA/L and 80 mg 2,4-D/L gave the highest percentage of callus induction (88%) as…

بواسطة SALEH ALANSI, FAHAD AL-QURAINY, MOHAMMAD NADEEM, SALIM KHAN*, ABDEL-RHMAN Z. GAAFAR, MOHAMED TARROUM, AREF ALSHAMERI
2018
تم النشر فى:
Pak. J. Bot