Cryopreservation: A tool to conserve date palm in Saudi Arabia
The cryostoring of embryogenic tissue of the date palm (Phoenix dactylifera L. cv. Sagai) was examined
through dehydrated-encapsulation, vitrification, and vitrification-encapsulation. The most extreme
regeneration rate (53.33%) of epitomized, cryostored liquid nitrogen (+LN) treated embryos was observed
when pre-embryonic masses were hatched with 0.5 M sucrose for 48 h pursued by 6 h air drying out. The
most noteworthy survival rate (80.0%) of epitomized, cryopreserved embryonic cluster came about when
calli were hatched with 0.3 or 0.7 M sucrose for 48 h pursued by four hours of lack of hydration, or with
0.5 M sucrose for 48 h without air drying out or with 2 h of air drying out. Following cryopreservation
utilizing the embodiment vitrification convention, the most astounding survival (86.7%) as well as the
greatest growth (46.7%) was accomplished when the typified vitrified, cryopreserved calli were treated
with Vitrification Solution 2 for plants (PVS2) for 60 min at 25 C. Cryopreservation utilizing the vitrification
convention brought about the most extreme recuperation of 53.3%, when vitrified-cryopreserved
calli were subjected to PVS2 solution for 30 min at 25 C. Most extreme (40%) regeneration of vitrified,
cryopreserved embryonic calli was seen when these calli were treated with PVS2 solution for 60 min
at 25 C. The outcome got amid this investigation of regrowth after cryopreservation of the cv. Sagai
was over the base suitable for a cryo-germplasm bank. Recovery and regrowth were above 30% for all
the techniques developed for the cv. Sagai
