Naringin inhibits the biofilms of metallo-β-lactamases (MβLs) producing Pseudomonas species isolated from camel meat
M., Husain, F. . 2020
Food producing animals harbouring bacteria carrying drug resistance genes especially the metallo-betalactamase
(MBL) pose high risk for the human population. In addition, formation of biofilm by these drug
resistant pathogens represents major threat to food safety and public health. In this study, metallo-blactamases
(MbLs) producing Pseudomonas spp. from camel meat were isolated and assessed for their
biofilm formation. Further, in vitro and in silico studies were performed to study the effect of flavone naringin
on biofilm formation against isolated Pseudomonas spp. A total of 55% isolates were found to produce
metallo-b-lactamase enzyme. Naringin mitigated biofilm formation of Pseudomonas isolates up to
57%. Disturbed biofilm architecture and reduced the colonization of bacteria on glass was observed under
scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The biofilm related
traits such as exopolysaccharides (EPS) and alginate production was also reduced remarkably in the presence
of naringin. Eradication of preformed biofilms (32–60%) was also observed at the respective
0.50 MICs. Molecular docking revealed that naringin showed strong affinity towards docked proteins
with binding energy ranging from 8.6 to 8.8 kcal mol1. Presence of metallo-b-lactamase producers
indicates that camel meat could be possible reservoir of drug-resistant Pseudomonas species of clinical
importance. Naringin was successful in inhibiting biofilm formation as well as eradicating the preformed
biofilms and demonstrated strong binding affinity towards biofilm associated protein. Thus, it is envisaged
that naringin could be exploited as food preservative especially against the biofilm forming foodborne
Pseudomonas species and is a promising prospect for the treatment of biofilm based infections.
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