ZOO 455 (Practical)

Cell Culture: Handling of Cell Culture, Sub-culturing of Cells

DNA Isolation: Preparation of Buffer and Reagents, Cell Lysis and DNA Isolation, Purification of DNA, Quantification of DNA

Polymerase Chain Reaction (PCR): Concept of PCR and Primer Design, Master mix preparation, Programming of PCR, Amplification of DNA by PCR

RNA Isolation: Buffer and Reagents Preparation for RNA Isolation, Lysis of Cells and RNA Isolation, Purification of RNA, Quantification of RNA of Nanodrop

DNA Gel electrophoresis: Basics of DNA Gel Electrophoresis, Preparation of Buffers for Electrophoresis, Gel Loading of DNA Samples, Staining and Visualization of DNA

RNA Gel electrophoresis: Preparation of Buffers for RNA Electrophoresis, Gel Loading of RNA Samples, Staining and Visualization of RNA

Synthesis of Complementary DNA (cDNA): Preparation of cDNA Master mix, PCR Programming for cDNA Synthesis, Amplification of cDNA

Real Time PCR (qPCR): Programming of Light Cycler qPCR Machine, Master mix Preparation for qPCR, Amplification of Genes by qPCR.

Quantification of Differential Expression of Genes: Data Mining of Amplified qPCR, Genes Fold-up and down, Regulation of Amplified, Genes Data Representation Method

Analyzing Genes Sequences Using BLAST (Bio-informatics) Tool: Designing of Primers, Similarity Index of DNA or RNA Using BLAST