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الأمجد الجيلاني منصور

Professor

أستاذ دكتور

كليات العلوم
قسم علم الحيوان, مبني 5 مكتب رقم 113 الطابق الأرضى
المنشورات
مقال فى مجلة
2021

Nitric oxide synthase potentiates the resistance of cancer cell lines to anticancer chemotherapeutics

Exploration of NO Mechanismsin Anticancer Chemotherapeutic Resistance

Abstract:
Background: Despite the advancement in the fields of medical science and molecular biology, cancer is still the leading cause of death worldwide. Chemotherapy is a choice for treatment; however, the acquisition of chemoresistance is a major impediment for cancer management. Many mechanisms have been postulated regarding the acquisition of chemo-resistance in breast cancer and the impact on cellular signalling and the induction of apoptosis in tumour cells. The mechanism of the apoptotic mutation ofp53 and bcl-2 proteins is commonly associated with increased resistance to apoptosis and, therein, to chemotherapy.

Objectives: The current study was aimed to investigate A172 and MDA-MB-231 cancer cells’sensitivity against chemotherapeutic drugs, including cisplatin, doxorubicin, and paclitaxel with different doses. Moreover, it estimates resistance of cancer cells by evaluating Nitric Oxide Synthase (NOS) expression and evaluate its correlation with the expression profile proteins of the apoptosis regulating Bcl-2 family.

Methods: Dose-dependent sensitivity to cisplatin, doxorubicin or paclitaxel was evaluated on spheroid cultured A172 and MDA-MB-231 cells lines, was measured by time-lapse microscopy over a 72h period. Expressions of two Nitric Oxide (NO) synthases isoforms (iNOS, eNOS), anti-apoptotic (Bcl-2, phospho-Bcl-2, Mcl-1, and Bcl-xL) and proapoptotic (BID, Bim, Bok, Bad, Puma, and Bax) were evaluated by Western blot. The effect of NO modulation on antiand pro-apoptotic molecule expression was also studied using Western blot.

Results: A172 cells show more resistance to chemotherapy drugs than MDA-MB-231 cancer cells, therefore, they need higher doses for apoptosis. Resistance of gliomas might be returned to higher significant expression of endothelial eNOS expression. It was clear that there is not a significant effect of NO modulation on the expression of pro- andantiapoptotic proteins on both cell lines.

Conclusion: The present work provides a putative mechanism for the acquisition of drug resistance in breast cancer and glioma, which might be significant for clinical outcomes.

نوع عمل المنشور
Article
اسم الناشر
Bentham Science
رقم المجلد
--
مجلة/صحيفة
Anti-Cancer Agents in Medicinal Chemistry
الصفحات
1-10
مزيد من المنشورات
publications

Abstract
Purpose

بواسطة Abdel-Baki1AA, Abdel-Haleem HM, Abdel-Tawab A, Al-Quraishy S, Mansour L
2021
تم النشر فى:
springer
publications

Abstract

بواسطة Alkhuriji AF., AlOmar SY., Babay ZA, El-khadragy MF, Alsharidah R, Alharbi H,a1 Alnafjan AA, Mansour L
2021
تم النشر فى:
Kanger