BCH 303 (Protein Biochemistry)

Topics Covered

Introduction

 

Amino acids

Definitions and types of amino acids

Functions of amino acids

Properties of amino acids: (Polarity, Stereoisomers, Light absorption, Ionization)

 

Structure & Classification of standard amino acids

Functional groups in amino acids

Introduction of Protein

Peptide Bond (formation, structure, & properties), and terminology: amino acids versus residue versus polypeptide & proteins

Protein structure (primary, secondary, tertiary, and quaternary)

Protein denaturation

 

 

Structural classification of proteins: (Fibrous proteins and Globular proteins: representatives of all-alpha, all-beta, and alpha/beta proteins)

 

 

 

Protein function

Functional classifications: enzymes, immunoglobulins, transport (O2, fatty acids), regulatory (hormones etc), structural, & movement, with examples.

complex proteins (metal ions, cofactors, lipids, carbohydrates, etc)

Introduction to enzymes

Enzyme mechanism:

Factors affecting reaction velocity of enzymes

 

peptides

Proteins general methods

Protein purification

Protein quantification

Amino Acid Analysis

Protein Sequencing

Mass Spectrometry and Proteomics