BCH 303 (Protein Biochemistry)

Topics Covered

Introduction

 
Amino acids
Definitions and types of amino acids
Functions of amino acids
Properties of amino acids: (Polarity, Stereoisomers, Light absorption, Ionization)
 

Structure & Classification of standard amino acids
Functional groups in amino acids

Introduction of Protein

Peptide Bond (formation, structure, & properties), and terminology: amino acids versus residue versus polypeptide & proteins
Protein structure (primary, secondary, tertiary, and quaternary)
Protein denaturation
 

 
Structural classification of proteins: (Fibrous proteins and Globular proteins: representatives of all-alpha, all-beta, and alpha/beta proteins)
 
 

 
Protein function
Functional classifications: enzymes, immunoglobulins, transport (O2, fatty acids), regulatory (hormones etc), structural, & movement, with examples.
complex proteins (metal ions, cofactors, lipids, carbohydrates, etc)
Introduction to enzymes
Enzyme mechanism:
Factors affecting reaction velocity of enzymes
 

peptides

Proteins general methods
Protein purification
Protein quantification
Amino Acid Analysis
Protein Sequencing
Mass Spectrometry and Proteomics