A reverse complementary multimodal imaging system to visualize microRNA9-involved neurogenesis using peptide targeting transferrin receptor-conjugated magnetic fluorescence nanoparticles
SH, Jo, MH; Lee, Ali,BA; AlKhedhairy, AA; Lee, CH; Kim, BJ; Haam, SG; Huh, YM; Ko, HY; Kim, . 2012
Multimodal imaging systems may eliminate the disadvantages of individual imaging modality by
providing complementary information about cellular and molecular activites. In this sutdy, we developed
a reverse complementary multimodal imaging system to image microRNAs (miRNA, miR) during neurognesis
using transferrin receptor (TfR) and a magnetic fluorescence (MF) nanoparticle-conjugated
peptide targeting TfR (MF targeting TfR). Both in vitro and in vivo imaging demonstrated that, in the
absence of miR9 during pre-differentiation of P19 cells, the MF targeting TfR nanoparticles greatly targeted
TfR and were successfully internalized into P19 cells, resulting in high fluorescence and low MR
signals. When the miR9 was highly expressed during neurogenesis of P19 cells, the MF targeting TfR
nanoparticles were hardly targeted due to the miR9 function, which represses the expression and
functional activity of TfR from the miRNA TfR reproter gene, resulting in low fluorescence and high MR
signals. The reverse complementary multimodal miRNA imaging system may serve as a new imaging
probe to montior miRNA-involved cellular developments and diseases.
Recently, nanoscale (<100 nm) inorganic materials, especially spherical shaped zinc oxide quantum dots
(ZnO-QDs), have received a lot of attention from the broad community because of…