BCH 320 General Enzymes

Course Description:
General aspects: nature of enzymes, localization, units of enzyme activity, specificity and specific
Nonenzyme catalysts: ribozymes and apoenzymes. Enzymes kinetics. Michaelis-Menten equation.
Enzyme inhibition. Irreversible inhibition and suicide inhibition. Bisubstrate reaction. Cooperativity
and allosteric enzyme. The transition state theory and its analogs. Types of enzymatic catalysis.
Definition, classification of coenzymes. Multi-molecular form of enzymes. Isolation, purification,
characterization of enzymes and criteria of purity of enzymes. Applications of free and immobilized
enzymes in the food, and pharmaceutical industries. Enzyme applications in cheese manufacture.
Utilization of enzymes baking. Analytical and therapeutic applications of enzymes.