453 كلن وراثة طبية و جزيئية CLS 453 Medical and Molecular Genetics
يقدم هذا المقرر دراسة مركزة عن التركيب والوظائف البيولوجية للخلايا والأحماض النووية وعملية تخليق والبروتينات وطرق التحكم فيها في الخلية الأولية والمتقدمة بالإضافة إلى نبذة عن الطفرة الجينية والهندسية والوراثية والتطبيقات العملية في التحاليل المخبرية والأمراض الوراثية .
This course is designed to give a basic understanding of the theoretical and practical aspects of medical genetics. In addition to molecular medical genetics, the course includes a study of cytogenetics, DNA, RNA, mutation, mutation detection techniques, PCR, cloning, population genetics and clinical genetics. In the practical part of the course, different molecular techniques used in medical genetics are studied and include gel electrophoresis, DNA extraction, PCR, and bioinformatics.
CLS 453: Lectures Outline
Weeks Subjects
1. History of Genetics
2. The Cell
3. Cytogenetics (Chromosomes)
4. DNA Structure and Function
5. Mutation
6. Mutation Detection Techniques
7. PCR
8. Cloning
9. Population Genetics
10. Clinical Genetics and Genetic Disorders
11. Chromosomal disorders
12. Single gene disorders
13. Sickle Cell Anaemia & Thalassemia
CLS 453: Laboratory Schedule
Weeks Subjects
1. Safety and Precautions in pre-PCR, PCR and post-
PCR: avoiding contamination; how to handle DNA
samples
2. Buffer and Electrophoresis:
- TAE Stock Buffer
- TBE Stock Buffer
- Agarose Gel
- Polyacrylamide Gel (PAGE)
- Stains
3,4. DNA Extraction
RNA Extraction
Tissue Extraction
5. PCR Amplification
Design Primers
Reagents to be used (Kit)
DNA Marker (Ladder)
6. Bioinformatics:
- Database of Genes
- Gene Bank
- Gene Map
7-13. Technique used after PCR
- Mutation Detection
- SSCP
- DHPLC
- Restriction Enzymes
- ARMS
- ASO
- Sequencing
Assessments:
First Mid Term Examination: 20
Second Mid Term Examination: 20
Final Practical Examination: 20
Final Theoretical Examination: 40
انظر نموذج الاختبار المرفق
see the attached exam sample