تجاوز إلى المحتوى الرئيسي
User Image

Dr. Abdulwahed Fahad Alrefaei

Associate Professor

Faculty member in Ecology and Molecular Epidemiology

كلية العلوم
Zoology Department, College of Sciences, ground floor, Office No. AB 58
المنشورات
مقال فى مجلة
2021

Organochlorine pesticides negatively influenced the cellular growth, morphostructure, cell viability, and biofilm-formation and phosphate-solubilization activities of Enterobacter cloacae strain EAM 35

, Shahid M., Manoharadas S., Altaf M., Alrefaei A. . 2021

An in vitro study was conducted to assess the impact of organochlorine pesticides (OCPs) on cellular growth, morphology, cell viability, biofilm-formation activity, and growth-regulating substances of a soil bacterium. Phosphate-solubilizing EAM 35 isolated from rhizosphere soil was molecularly identified as Enterobacter cloacae (accession number MT672578.1). Strain EAM 35 tolerated varying levels of OCPs, viz., benzene hexachloride (BHC), chlorpyrifos (CP), dieldrin (DE), and endosulfan (ES). The toxicity of OCPs to strain EAM 35 was displayed in a concentration-dependent manner. Among the OCPs, ES at a concentration of 200 μM showed a higher toxicity, where it maximally reduced the bacterial synthesis of indole-3-acetic acid (IAA), salicylic acid (SA), and 2,3-dihydroxy-benzoic acid (DHBA) by 73% (p ≤ 0.001), 85% (p ≤ 0.005), and 83% (p ≤ 0.001), respectively, over the control. While comparing the toxicity of OCPs to P-solubilizing activity of E. cloacae after 10 days of growth, the toxicity pattern followed the order ES (mean value = 82.6 μg mL-1) > CP (mean value = 93.2 μg mL-1) > DE (mean value = 113.6 μg mL-1) > BHC (mean value = 127 μg mL-1). Furthermore, OCP-induced surface morphological distortion in E. cloacae EAM 35 was observed as gaps, pits on both cellular facets, and fragmented and disorganized cell structure under a scanning electron microscope (SEM). The membrane-compromised cells increased as the concentrations of OC pesticides increased from 25 to 200 μM. Additionally, microbial counts (log10 CFU/mL) were also affected after pesticide exposure and decreased with increasing concentrations. While assessing the impact of OCPs on inhibition (%) of log10 CFU/mL, 150, 175, and 200 μM concentrations of ES completely reduced the growth of E. cloacae. Similarly, while comparing the toxicity of higher concentrations of OCPs to bacterial growth, sensitivity followed the order ES > DE > CP > BHC. In addition, the biofilm-formation ability of strain EAM 35 was inhibited in a pesticide-dose-dependent manner, and it was statistically (p ≤ 0.05, p ≤ 0.005, and p ≤ 0.001) significant. Conclusively, the present study clearly suggests that before applying pesticides to soil, their recommended dose should carefully be monitored.

رقم المجلد
6
مجلة/صحيفة
ACS Omega
الصفحات
5548-5559
مزيد من المنشورات
publications

The current study aimed to examine thymic stromal lymphopoietin receptor (TSLPR) genetic variation and breast cancer (BC) susceptibility in women in Saudi Arabia.

بواسطة SEMLALI A., ALMUTAIRI M., ALHARBI S., AL AMRI A., ALREFAEI A. F., ALMUTAIRI B., & ROUABHIA M.
2021
publications

Methylenetetrahydrofolate reductase (MTHFR) polymorphism plays a fundamental role in susceptibility to various diseases, including cancers and autoimmune diseases.

بواسطة ALMUTAIRI M., AL-NUMAIR, N., PARINE, N., ALMUTAIRI, B., ALREFAEI, A., ROUABHIA M., SEMLALI, A.
2021
publications

Camptothetin (CPT) is a quinoline alkaloid originally isolated from the Chinese tree, Camptotheca acuminata Decne. CPT was found to have anticancerous and antiviral properties.…

بواسطة DEVASIA R., ALTAF M., ALREFAEI A., MANOHARADAS S.
2021