Genotoxicity in the freshwater gastropod Lymnaea luteola L: assessment of cell type sensitivities to lead nitrate

مقال فى مجلة
SA, Daoud Ali, Huma Ali, Saud Alarifi, Ali Al-Amer, P.G. Kumar, Sudhir Kumar, Agha P. Masih, Saad Alkahtani, A A. Alkahtane, . 2017
رقم الانشاء
NO. 2,
رقم المجلد
VOL. 33,
تاريخ المؤتمر
ملخص المنشورات

The aquatic ecosystems are converting into the highly contaminated site due to environmental pollutants. The present study explores the oxidative stress and toxic potential of lead nitrate in freshwater snail Lymnaea luteola (L. luteola) L. The snails were exposed to an environmentally relevant concentration of lead nitrate for 24 and 96 h. Later exposure to lead nitrate (0, 10, 20 and 40 µg/mL) to the freshwater snail, the level of reactive oxygen species, malondialdehyde and nitric oxide (NO) were increased and glutathione, glutathione-S-transferase were decreased. Lead-nitrate-induced haemocyte cell death and it was observed by using Annexin-V FITC/PI through a flow cytometer. DNA damage in haemocyte cells was measured at above doses of lead-nitrate exposure for 24 and 96 h and it was compared to the untreated snail. Average tail DNA (%) and olive tail moment in single-cell gel test were increased dose and duration fashion and maximum DNA damage was measured at 96 h. These results indicate the potential toxicity and genotoxicity of lead nitrate in acute treatment to L. luteola and single-cell gel test are the assay for rapid detection of genetic effects.

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