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Zahid Abu Talib Khan

Assistant Professor

Assistant Professor, Research

كلية العلوم
AA13 Department of Biochemistry, College of Science, Building 5, King Saud University, Riyadh 11451
السيرة الذاتية

CV

نص السيرة الذاتية




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Zahid Khan, Ph.D.

Dept. of Biochemistry, College of Science

King Saud University, Riyadh, KSA

Email: zahidatkhan@yahoo.com

Education

Doctor of Philosophy

Laboratory of Cancer Genes, Cancer Research Institute, Tata Memorial Center, Mumbai (Affiliated to Bombay University, Mumbai, India)

1997-2002

Master of Science (Biochemistry) from Aligarh Muslim University, India

1994-1996

Bachelor of Science (Biochemistry) from Aligarh Muslim University, India

1991-1994

 

 

Research interest

My overall research goal is to understand the mechanisms by which cancers evolve and progress. Specifically, I am interested in delineating the pathways and the molecules that are involved in the development and invasion/metastasis of cancer with an ultimate goal of targeting these pathways and molecules for therapy. I also find it intriguing to investigate how cancers adapt and evolve to acquire resistance towards therapy and to develop strategies to overcome drug resistance including examining the involvement of cancer stem cells in this resistance.

Currently, I am involved in several different projects related to cancer, including deregulation of Wnt signaling pathways in breast and colorectal cancers, the most prevalent cancers in Saudi Arabia. The Wnt signaling pathway has been causally involved in the pathogenesis of several different cancers and has a role in the maintenance of stem cells. Thus, studying this pathway would also provide an opportunity to gain insights into the role of stem cells in the development of cancers.

 

Research Experience [Area of specialization - Cancer research]

 

·         Assistant Professor: December, 2008 – till date

Department of Biochemistry, College of Science, King Saud University, Riyadh- 11451, KSA.

o   Wnt signaling in breast and Colorectal cancers.

o   Understand the molecular pathogenesis of breast and Colorectal cancers in Saudi patients.

Research Laboratory set-up

o   Cell culture unit

o   Immunohistochemistry lab

Research Grant

o   Elucidation of molecular pathogenesis and classification of Breast cancers based on gene-

     expression patterns to individualize therapy in Saudi patients. (Grant # 09-BIO897-02 from King 

     Abdul Aziz City for Science and Technology, Saudi Arabia). Amount- US dollars 502,000

     May 2011-April 2013

o   Designing theranostic aptamers for Bcl-2 inhibition and phosphatidylserine detection to target apoptosis in cancer treatment. CO-I (Grant # 12-MED2670-02 from King Abdul Aziz City for Science and Technology, Saudi Arabia). Amount- US dollars 500,000 2013-2015.

 

·         Senior Scientist: January, 2008 – October, 2008

SibTech, Inc., 115 Commerce Drive, Brookfield, CT-06804

o   Targeting anti-apoptotic drugs to failing cardiomyocytes.

o   Targeted delivery of Anthrax inhibitors.

 

·         Postdoctoral fellow:January, 2007 - December, 2007; Preceptor: Dr. Anna Bafico

Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY-10029

o   Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor (Mol. Cell. Biol. 2007 October; 27(20): 7291-7301)

o   Identification of the molecular mechanisms responsible for establishing autocrine Wnt signaling in breast/ovarian tumor cell lines.

o   Study the influence of autocrine Wnt signaling on the transformed phenotype of human breast and ovarian cancer cells.

 

·         Postdoctoral fellow: August, 2002 - January, 2007; Preceptor- Prof. Stuart Aaronson

Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY-10029

o   Investigation of mechanisms involved in human tumorigenesis. [Cancer Research 2008 Mar 1; 68(5): 1417-26]

Contribution: Generated bi and tricistronic retroviral vectors for co-expression of genes and participated in the characterization of an in vitro human cell transformation model generated in the lab by introducing specific cellular oncogenes to delineate gene/pathway abrogations in neoplastic conversion of normal human cells.

o   Investigation of endogenous keratinocyte growth factor (KGF) as a paracrine acting mediator in the growth and survival of KGFR expressing tumor cells.

Key achievement:  Demonstrated the tumor suppressive effect of KGFR.

o   Investigate the regulation and functions of p53 in hypoxic stress.

Contribution: - Performed experiments, data analysis and participated in discussions for a study demonstrating that low oxygen environment protects cells against p53-induced apoptosis. [J Biol Chem 2010 April 30; 285(18): 13658-65]

 

·         Doctoral Research: November, 1997 - August, 2002; Preceptor- Dr. Dhananjaya Saranath

Laboratory of Cancer Genes, Cancer Research Institute, Tata Memorial Center, Mumbai, India

o   Investigate the molecular pathogenesis of cervical cancer. (Gynecol Oncol. 2002 Aug; 86(2): 157-62)

Key achievements:  Estimated the prevalence of Human papillomavirus (HPV) 16/18 in cervical premalignant lesions and cervical cancer and identified correlation of p53 polymorphism at codon 72 and higher risk for developing HPV16/18-associated cervical carcinomas.

 

Technical Expertise

·         Extensive experience and expertise in a wide range of techniques important for cancer research including Molecular Biology, Biochemistry, Cell Biology and Immunohistochemical techniques.

 

Other Experience

·         Trained graduate students and research assistants

·         Trained personnel and helped to set up a high-throughput HPV screening laboratory supported by Bill and Melinda Gates Foundation in Barshi, India.

 

Honors/Awards

·         Research Fellowship and pre-doctoral grant from Council of Scientific and Industrial Research - National Eligibility Test (CSIR-NET) (1997-2002).

·         Graduate Aptitude Test in Engineering (GATE) – For Life Sciences (1996-National level, Percentile score: 90.19).

 

Publications

 

1.            Alanazi MS, Parine NR, Shaik JP, Alabdulkarim HA, Ajaj SA, Khan Z*. Association of single nucleotide polymorphisms in Wnt signaling pathway genes with breast cancer in Saudi patients. PLoS ONE 8(3): e59555. doi:10.1371/journal.pone.0059555. * Corresponding author.

2.            Saeed HM, Alanazi MS, Abduljaleel Z, Al-Amri A, Khan Z. Molecular cloning, sequence analysis and expression in Escherichia coli of Camelus dromedarius glucose-6-phosphate dehydrogenase cDNA. Protein Expr Purif. 2012 Jun;83(2):190-7.

3.            Alanazi M, Abduljaleel Z, Khan W, Warsy AS, Elrobh M, Khan Z, Al Amri A, Bazzi MD. In silico analysis of single nucleotide polymorphism (SNPs) in human β-globin gene. PLoS One. 2011; 6(10):e25876. Epub 2011 Oct 20.

4.            Samantha Carrera, Petra J. de Verdier, Zahid Khan, Bo Zhao, Alka Mahale, Karen J. Bowman, Muri Zainol, George D. D. Jones, Sam W. Lee, Stuart A. Aaronson and Salvador Macip. Protection of cells in physiological oxygen tensions against DNA damage-induced apoptosis. J Biol Chem 2010 April 30; 285(18): 13658-65.

5.            Alka M. Mahale, Zahid A.T. Khan, Makoto Igarashi, Gouri J. Nanjangud, Rui Fang Qiao, Shen Yao, Sam W. Lee, Stuart A. Aaronson. Clonal selection in malignant transformation of human fibroblasts transduced with defined cellular oncogenes. Cancer Research 2008 Mar 1; 68(5): 1417-26
6.            Khan Z, Vijayakumar S, Villanueva de la Torre T, Rotolo S and Bafico A. Analysis of endogenous LRP6 function reveals a novel feedback mechanism by which Wnt negatively regulates its receptor. Mol. Cell. Biol. 2007 October; 27(20): 7291-7301.
7.            Saranath D, Khan Z, Tandle AT et al. HPV 16/18 prevalence in cervical lesions/cancers and p53 genotypes in cervical cancer patients from India. Gynecol Oncol. 2002 Aug; 86(2): 157-62.
8.            Khan Z (2000). HPV in cervical cancer. Indian Association for Cancer Research, Newsletter, vol 16(1) 10-11. 
9.            Saranath D, Khan Z, Shrivastava SK et al. Prevalence of HPV 16/18 and p53 polymorphisms in cervical cancers in Indian women. In: Recent Developments in the Management of Cancer of Uterine Cervix, Eds. Gupta S and Sood OP, Proc. Sixth Ann. Ranbaxy Sci. Foundation, (1999) 57-63.