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Mohamed Ali Farrag

Associate Professor

علم الفيروسات الطبية

كلية العلوم
مبنى (5)-الدور الأرضي-أ ب 44 (AB44)
المنشورات
مقال فى مجلة
2014

Group B strains of human respiratory syncytial virus in Saudi Arabia: molecular and phylogenetic analysis

The genetic variability and circulation pattern of human respiratory syncytial virus group B (HRSV-B) strains, identified in Riyadh during the winters of 2008 and 2009, were evaluated by partial sequencing of the attachment (G) protein gene. The second hypervariable region (HVR-2) of G gene was amplified by RT-PCR, sequenced and compared to representatives of different HRSV-B genotypes. Sequence and phylogenetic analysis revealed that all Saudi strains belonged to the genotype BA, which is characterized by 60-nucleotide duplication at HVR-2. Only strains of 2008 were clustered with subgroup BA-IV, while those isolated at 2009 were clustered among the most recent subgroups (particularly BA-X and CB-B). Amino acid sequence analysis demonstrated 18 amino acid substitutions in Saudi HRSV-B strains; among which five are specific for individual strains. Furthermore, two potential N-glycosylation sites at residues 230 and 296 were identified for all Saudi strains, and an additional site at amino acid 273 was found only in Riyadh 28/2008 strain. O-glycosylation was predicted in 42-43 sites, where the majority (no = 38) are highly conserved among Saudi strains. The average ratio between non-synonymous and synonymous mutations (ω) implied stabilizing selection pressure on G protein, with evidences of positive selection on certain Saudi strains. This report provides preliminary data on the circulation pattern and molecular characteristics of HRSV-B strains circulating in Saudi Arabia.

اسم الناشر
Virus Genes
مزيد من المنشورات
publications

With the emergence of SARS-CoV-2, routine surveillance combined with sequence and phylogenetic analysis of coronaviruses is urgently required.

بواسطة Alamri, K.A.; Farrag, M.A.; Aziz, I.M.; Dudin, G.A.; Mohammed, A.A.; Almajhdi, F.N.
تم النشر فى:
Viruses-Basel
publications

Background: Equine influenza is an important cause of respiratory disease in equids. The causative virus; EIV, is highly variable and can evolve by accumulation of mutations, particularly in the…

بواسطة Ahmed, Basem Mohamed; Bayoumi, Mahmoud Mohamed; Farrag, Mohamed Ali; Elgamal, Aly; Daly, Janet Mary; Amer, Haitham Mohamed
2022
تم النشر فى:
Virology Journal
publications

Pure PANI and TiO2/PANI nanocomposite composites with widely different TiO2 weight percentages were synthesized by chemical oxidative method. Polymerization of aniline using ammonium per sulfate (…

بواسطة J. Kalaiarasi, D. Balakrishnan, Lamya Ahmed Al-Keridis, Fahd A. Al-mekhlafi, Mohamed A. Farrag, C. Chenthis Kanisha, Manavalan Murugan, C. Pragathiswaran
2022