Comparative study on the independent and combined effects of omega-3 and vitamin B12 on phospholipids and phospholipase A2 as phospholipid hydrolyzing enzymes in PPA-treated rats as a model for autistic traits

Journal Article
El-Ansary, Hanan Alfawaz, Ramesa Shafi Bhat, Manar Al-Mutairi, Osima M. Alnakhli, Abeer Al-Dbass, Mona AlOnazi, Majidh Al-Mrshoud, Iman H. Hasan and Afaf . 2018
نوع عمل المنشور: 
المجلة \ الصحيفة: 
Lipids in Health and Disease
رقم العدد: 
رقم الإصدار السنوي: 
مستخلص المنشور: 

Background: Abnormal phospholipid metabolism is a major component of many neurodevelopmental disorders

including autism. Oral administration of propionic acid (PPA) can produce behavioral abnormalities and biochemical

features in rodents similar to those observed in autism and can thus be used as a model to understand impaired

brain fatty acid metabolism in autism.

Methods: The present study was designed to understand alterations in phospholipid metabolism in the brain of a

rodent model of autism and to explore omega-3 and vitamin B12 as remedies. Five groups of rats were selected:

Group 1 was the control. Group 2 was the rodent model of autism treated with a neurotoxic dose of PPA. Group 3

was given vitamin B12 cobalamin (16.7 mg/kg/day) for 30 days after PPA treatment. Group 4 was given pharmaceutical

grade Omega-3 (200 mg cholesterol free-DHA/kg body weight/day), a product of Madre lab, Germany, for 30 days

after PPA treatment for 3 days. Group 5 was given a combined dose of ω-3 + Vitamin B12 for the same duration post-

PPA treatment. Phospholipid levels and Phospholipase A2 were measured in the brain homogenates of all the groups.

ELISA and western blotting were used to detect the cPLA2 protein level.

Results: A significant decrease in phospholipid levels and a significant increase in cPLA2 were found in brain tissue of

PPA-treated rats; however, both ω-3 and vitamin B12 were efficient in ameliorating the neurotoxic effect of PPA.

Conclusion: Both ω-3 and vitamin B12 may play a role in ameliorating impaired phospholipid metabolism in autism;

however, proper clinical trials are needed.

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