Effect of Centella asiatica Formulation on the Pharmacokinetics of Amitriptyline in Rats: A Herb-Drug Interaction Study

Journal Article
, Fowad KHURSHID, Jeyabalan GOVINDASAMY, Habibullah KHALILULLAH, Mohammed Shivli NOMANI, Mudassar SHAHID , Md Ruhal AIN & Mohammad S. ALSULTAN . 2018
Magazine \ Newspaper: 
Lat. Am. J. Pharm.
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SUMMARY. This study evaluates the effect of Centella asiatica formulation on the pharmacokinetics of amitriptyline in rats. In this study, rats were randomly divided into two groups (n = 6 each) which were served as a control (amitriptyline alone) and treatment group (amitriptyline with C. asiatica), respectively. Rats were administered vehicle saline or C. asiatica (62.5 mg/kg, p.o. daily for 7 days), then administered a single amitriptyline dose (25 mg/kg, p.o.) on day 8. Plasma samples were analyzed for amitriptyline concentration using a developed and validated reversed-phase high-performance liquid chromatography (RPHPLC) method. Pharmacokinetic parameters were calculated using non-compartmental analysis. The coadministration of C. asiatica with amitriptyline resulted in increased plasma maximum concentration (Cmax), area under the curve (AUC0–24) and elimination half-life (t1/2) by 14.34, 23, and 13.81%, respectively. The present study demonstrated that C. asiatica extract has the potential to alter the pharmacokinetics of amitriptyline by a significant reduction in amitriptyline clearance and an increase in its AUC, Cmax and t1/2.