Synthesized Chitosan-Sodium Alginate- Polyethylene glycol-D-Pinitol nanocomposites showed antiarthritic activity on Freund’s Complete Adjuvant-induced arthritis in rats

Journal Article

2022

Published in: https://doi.org/10.1016/j.arabjc.2021.103592

Shenqiang Qiu, Arunachalam Chinnathambi , Saleh H. Salmen, D.S. Prabakaran, Sulaiman Ali Alharbi , Vishnu Priya Veeraraghavan , Krishna Mohan Surapaneni

Background: Osteoarthritis is a severe degenerative joint disease characterized by swelling,
joint pain, degradation of cartilage extracellular matrix, synovitis, callus formation, and loss of
normal joint movements, which leads to functional limitations and seriously affect the patient’s life
quality.
Objective: The current research work was focussed to synthesize and characterize the chitosansodium
alginate-polyethylene glycol-d-pinitol nanocomposites (CSP-dP-NCs) and evaluate its antiarthritic
activity against the freund’s complete adjuvant (FCA)-triggered arthritis in animals.
Methodology: The CSP-dP-NCs were synthesized by standard method. The development and
properties of formulated CSP-dP-NCs were confirmed by several characterization techniques likeUV–visible, photoluminescence, X-ray diffractometer (XRD), Fourier Transform-Infrared
(FT-IR), scanning electron microscopic (SEM), energy dispersive X-ray (EDX), and dynamic light
scattering (DLS) analyses. The arthritis was induced in experimental rats via administering 0.1 ml of
FCA subcutaneously and then treated with 10 and 25 mg/kg of synthesized CSP-dP-NCs for
25 days. The bodyweight of animals were tabulated and then haematological parameters, organ
index, and paw volume was examined. The levels of liver marker enzymes were assessed by standard
techniques. The contents and pro-inflammatory cytokines and antioxidant parameters were quantified
using assay kits.
Results: The findings of characterization studies confirmed the development of CSP-dP-NCs
with size ranging from 180 nm, tetragonal appearance, and narrowed distribution. The CSP-dPNCs
treated arthritic animals demonstrated the improved bodyweight and haematological parameters.
The levels of thymus and spleen index and hind paw volume was decreased by the CSP-dPNCs.
The administration of CSP-dP-NCs to the arthritic rats also exhibited the decreased contents
of IL-6, IL-10, IL-1b, TNF-a, MDA levels and increased the GSH level, CAT and SOD activities.
The activities of ALP, ALT, and ASP also decreased in arthritic rats by the CSP-dP-NCs treatment.
Conclusion: Altogether, the findings of this study demonstrated the antiarthritic activity of formulated
CSP-dP-NCs against FCA-induced arthritic rats via modulating oxidative stress and
inflammatory parameters. Hence, it could be the effective drug for the arthritis treatment in the
future.

• Publication Work Type: بحث علمي
• Magazine \ Newspaper: Arabian Journal of Chemistry