Mushtaq Ahmad Ansari, PhD
Assistant Professor
College of Pharmacy
Department of Pharmacology and Toxicology
King Saud University
P.O. Box 2457, Riyadh 11451
Mobile no. 00966-536741024
E. mail: ansamushtaq@gmail.com; muansari@ksu.edu.sa  
 
 
 
 
 
To achieve excellence in the field of Molecular Biology and Biotechnology related research and always try to give our best to the particular institution.
 
 
 
 

Awarded PhD on (14 December 2010) entitled “Molecular Analysis of PIK3CA gene in Breast Cancer” under the supervision of Professor S. A. Husain in the Human Genetics Lab, Department of Bioscience, Jamia Millia Islamia, New Delhi-110025.
 
Master of Science (M.Sc.), Biotechnology from Department of Biotechnology, Barkatullah, University. Bhopal, India.
 
Bachelor of Science (B.Sc.) from Lucknow University, Lucknow, India.
 
 

 
 
 
 
 

• Working as Assistant Professor in Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia, (October 2011 - till date).

 

• Worked as a PhD scholar (July 2005 to 14 December 2010) entitled “Molecular Analysis of PIK3CA gene in Breast Cancer” under the supervision of Professor S. A. Husain in the Human Genetics Lab, Department of Bioscience, Jamia Millia Islamia, New Delhi-110025.

 

• Worked as a cytogenetist (23rd August -2004 to 11th June -2005) in Foundation for Research in Genetics and Endocrinology (FRIGE Reg. No: E/13237) 20/1. Bimanagar, Opp. Umiyavijay, Satellite Road, Ahmedabad-380015. Gujarat India.

 
 

 
 
 
 

Major responsibilities in MOLECULAR BIOLOGY include-

1.             Genomic & plasmid DNA isolation, purification and their estimation
2.             PCR, (Polymerase Chain Reaction)

1.             RNA isolation (from cells, tissues), purification & estimation
2.             cDNA preparation
3.             Real Time PCR
4.             Protein isolation (tissue and cells) and estimation by Lowry method

1.             Western blot

1.             SSCP (single strand conformation polymorphism)

1.             Electrophoresis
2.             Immunohistochemistry

1.             SDS PAGE

1.             Restriction Fragment length polymorphism (RFLP)

1.             Methylation specific PCR

 
Major responsibilities in Human Cytogenetics include -

1. Chromosomal analysis from blood, bone marrow, amniotic fluid, chorionic villi and abortus materials

1. Planting of samples, harvesting, slide making and banding of samples (GTG banding ,C banding, NOR banding)

1. Karyotyping

1. Sister Chromatid Exchanges (SCEs),

1. FISH (fluorescence in situ hybridization)

1. Diagnosis of pre-natal and post-natal diseases

 

 
 
 
 
 
 

• Hesham M. Korashy, Mushtaq A. Ansari, Zaid H. Maayah, Faisal Imam, Mohd Raish, Ibraheem M. Attaf, Naif O. Alharbi, Badr Moraished: Differential Effects of Sunitinib on the Expression Profiles of Xenobiotic Metabolizing Enzymes and Transporters in Rat Liver and Kidneys. Basic & Clinical Pharmacology & Toxicology 01/2016;  DOI:10.1111/bcpt.12555

 

• Imam F, Al-Harbi NO, Al-Harbia MM, Korashy HM, Ansari MA, Sayed-Ahmed MM, Nagi MN, Iqbal M, Khalid Anwer M, Kazmi I, Afzal M, Bahashwan S. Rutin Attenuates Carfilzomib-Induced Cardiotoxicity Through Inhibition of NF-κB, Hypertrophic Gene Expression and Oxidative Stress. Cardiovasc Toxicol. 2015 Dec 26.

 

• Faiyaz Shakeel, Gamal A Shazly, Mohammad Raish, Ajaz Ahmad, Mohd Abul Kalam, Naushad Ali, Mushtaq A Ansari, Gehan M Elosaily · Biological investigation of supersaturated self-nanoemulsifying drug delivery system of Piper cubeba essential oil. RSC Advances 01/2015; DOI:10.1039/C5RA22900F.  

 

• Sheikh Fayaz Ahmad, Khairy M.A. Zoheir, Mushtaq Ahmad Ansari, Ahmed Nadeem, Saleh A. Bakheet, Ali R. Al-Hoshani, Othman A. Al-Shabanah, Mohammed M. Al-Harbi, Sabry M. Attia. Histamine 4 receptor promotes expression of costimulatory B7.1/B7.2 molecules, CD28 signaling and cytokine production in stress-induced immune responses. Journal of Neuroimmunology 289 (2015) 30–42.

 
Ø  Mashooq A. Bhat, Mohamed A. Al-Omar, Mushtaq A. Ansari, Khairy M. A. Zoheir, Faisal Imam,  Sabry M. Attia, Saleh A. Bakheet, Ahmed Nadeem, Hesham M. Korashy, Andrey Voronkov. Design and synthesis of N-aryl-phthalimides as inhibitors of glucocorticoid- induced TNF receptor-related protein, pro-inflammatory mediators, and cytokines in carrageenan-induced lung inflammation. J Med Chem. 2015 Nov 25;58(22):8850-67.
Ø  Faisal Imam, Naif O. Al-Harbi, Mohammed M. Al-Harbi, Mushtaq Ahmad Ansari, Khairy M.A. Zoheir, Muzaffar Iqbal, Md. Khalid Anwer, Ali R. Al Hoshani, Sabry M. Attia, Sheikh Fayaz Ahmad. Diosmin downregulates the expression of T cell receptors, pro-inflammatory cytokines and NF-κB activation against LPS-induced acute lung injury in mice. Pharmacological Research, Volume 102, December 2015, Pages 1–11.

• Nadeem A, Al-Harbi NO, Al-Harbi MM, El-Sherbeeny AM, Ahmad SF, Siddiqui N, Ansari MA, Zoheir KM, Attia SM, Al-Hosaini KA, Al-Sharary SD. Imiquimod-induced psoriasis-like skin inflammation is suppressed by BET bromodomain inhibitor in mice through RORC/IL-17A pathway modulation. Pharmacol Res. 2015 Jul 3;99:248-257.

 
Ø  Sheikh Fayaz Ahmad, Khairy M.A. Zoheir, Mushtaq Ahmad Ansari, Hesham M. Korashy, Saleh A. Bakheet, Abdelkader E. Ashour, Sabry M. Attia. Stimulation of the histamine 4 receptor with 4-methylhistaminemodulates the effects of chronic stress on the Th1/Th2 cytokinebalance. Immunobiology.  2015 Mar;220(3):341-9.
Ø  Zaid H. Maayah, Hazem B. Ghebeh, Abdulqader A. Alhaider, Ayman O. El-Kadi, Anatoly A. Soshilov, Michael S. Denison, Mushtaq A. Ansari, and Hesham M. Korashy .Metformin inhibits 2, 7, dimethylbenz[a]anthracene-induced breast carcinogenesis and adduct formation in human breast cells by inhibiting the cytochrome P450 1A1/Aryl hydrocarbon receptor signaling pathway (Toxicology and Applied Pharmacology Toxicology and Applied Pharmacology 284 (2015) 217–226
Ø  Sheikh Fayaz Ahmada, Khairy M.A. Zoheir, Mushtaq Ahmad Ansari, Hesham M. Korashy, Saleh A. Bakheet, Abdelkader E. Ashour,Othman A. Al-Shabanah, Mohammed M. Al-harbi, Sabry M. Attia. The role of poly (ADP-ribose) polymerase-1 inhibitor in carrageenan-induced lung inflammation in mice.Mol Immunol. 2015 Feb; 63(2):394-405.

• Sheikh Fayaz Ahmad, Khairy M A Zoheir, Mushtaq Ahmad Ansari, Saleh A. Bakheet, Hesham M. Korashy, Abdelkader E. Ashour, Sabry M. Attia, Regulation of TNF-α and NF-kB activation through the JAK/STAT signaling pathway downstream of Histamine receptor-4 in a rat model of LPS-induced joint inflammation  Immunobiology 220 (2015) 889–898

 
Ø  Sheikh Fayaz Ahmad, Sabry M. Attia, Saleh A. Bakheet, Khairy M. A. Zoheir, Mushtaq Ahmad Ansari, Hesham M. Korashy, Hala E. Abdel-Hamied, Abdelkader E. Ashour, and Adel R. A. Abd-Allah. Naringin Attenuates the Development of Carrageenan-Induced Acute Lung nflammation Through Inhibition of NF-κb, STAT3 and Pro-Inflammatory Mediators and enhancement of IκBα and Anti-Inflammatory Cytokines. Inflammation. 2014 Aug 13. [Epub ahead of print].

• Hesham Mohamed Korashy. Hani A. Al-Suwayeh. Zaid H. Maayah.  Mushtaq Ahmad ansari Sheikh Fayaz Ahmad. Saleh A. Bakheet. Mitogen-Activated Protein Kinases Pathways Mediate the Sunitinib-Induced Hypertrophy in Rat Cardiomyocyte H9c2 Cells. Cardiovasc Toxicol. 2014 Jul 2. [Epub ahead of print].

 

• Zaid H. Maayah. Mushtaq Ahmad Ansari. Mohamed A. El Gendy.Mohammed N. Al‑Arifi Hesham M. Korashy. Development of cardiac hypertrophy by sunitinib in vivo and in vitro rat cardiomyocytes is influenced by the aryl hydrocarbon receptor signaling pathway. Arch Toxicol (2014) 88:725–738.

 

• Mushtaq A. Ansari, Zaid H. Maayah, Saleh A. Bakheet, and Hesham M. Korashy. The Role of Cytochrome P450 1A1 Signaling Pathway in Cardiotoxicity of Acute Lead Intoxication inVivo and in Vitro Rat Model. Toxicology 2013.01.024.

 

• Mohammad Raish, Mohsin Khurshid, Mushtaq Ahmad Ansari, Pankaj Kumar Chaturvedi, Su-Mi Bae, Jang Heub Kim, Eun Kyung Park, et al. Analysis of molecular cytogenetic alterations in uterine leiomyosarcoma by array-based comparative genomic hybridization. J Cancer Res Clin Oncol (2012) 138:1173-1186.

 

• Mohammad Raish, Varinderpal S. Dhillon,Arif Ahmad, Mushtaq Ahmad Ansari,Shahid Mudassar, Mohammad Shahid,Vineeta Batra, Pawan Gupta§,Bhudev Chandra Das, N.K. Shukla and Syed Akhtar Husain Promoter Hypermethylation in Tumor Suppressing Genes p16 and FHIT and Their relationship with Estrogen Receptor and Progesterone Receptor Status in Breast Cancer Patients from Northern India.Transl Oncol. 2009 December; 2(4):1–7.

 
 

 
 

• Sheikh Fayaz Ahmad, Khairy M A Zoheir, Mushtaq Ahmad Ansari, Saleh A.

 
 

• Ansari MA, Maayah ZH, Sheikh FA, Assiri MA, Al-Shamasan AI, Korashy HM sunitinib induces growth inhibition, cell cycle arrest, apoptosis, and oxidative stress in human breast cancer mcf7 cells. Toxicology and Applied Pharmacology (submitted).

 

• Saleh A. Bakheet,  Sheikh Fayaz Ahmad, Mushtaq Ahmad Ansari, Khairy M. A. Zoheir, Sabry M. Attiaa. β-1,3-Glucan mediates immunostimulation through induction of cell surface marker and cytokine secretion mechanisms in mice exposed to aflatoxin B1 (submitted).

 

• Sheikh Fayaz Ahmad, Khairy M A Zoheir, Mushtaq Ahmad Ansari, Saleh A. Bakheet, Ammar Cherkess Al Rikabi, Sabry M. Attia. The tyrosine kinase inhibitor tyrphostin AG126 reduces activation of inflammatory cells and increases Foxp3+ regulatory T cells during pathogenesis of rheumatoid arthritis. (submitted).

 

• Naif O. Al-Harbi, F. Imam, Mohamed M. Al-Harbi, Mushtaq Ahmad Ansari, Othman A. Al-Shabanah, Mohamed M. Sayed-Ahmed, Saleh A. Bahashwan Sheikh F. Ahmad. Dexamethasone attenuates LPS-induced acute lung injury through Inhibition of NF-kB, COX-2 and pro-Inflammatory mediators and augmentation of anti-Inflammatory Cytokine. (Submitted).

 
 

 
 
 
 

• Maayah ZH, Ansari MA, and Korashy HM (2013). Acute Lead Exposure Induces Cardiotoxicity in Vivo and in Vitro Rat Model through the Cytochrome P450 1A1 Signaling pathway. The 52nd Annual Meeting of the Society of Toxicology, 10-14 March, 2013, San Antonio, TX USA.

 

• Hesham M. Korashy, Ibraheem A. Attafi, Mohammed Assiri, Mushtaq Ahmad Ansari, Sheikh Fayaz Ahmad, Munthir M. Almujahid, Osama A. Almuzaini, Faisal A. Aldughaim, Farraj M. Alotaibi. Gefitinib, a tyrosine kinas inhibitor, induces cardiotoxicity and hypertrophy in rat cardiomyocyte H9c2 cells: role of oxidative stress and apoptosis. The 54thAnnual Meeting of the Society of Toxicology, 22-26 March, 2015, San Deigo, CA, USA

 

• Korashy, Hesham M.; Ansari, Mushtaq A; Maayah, Zaid H; Ahmad, Sheikh A; Assiri, Mohammed A; Al-Shamasan, Aws. Sunitinib induces growth inhibition, cell cycle arrest, apoptosis, and oxidative stress in human breast cancer mcf7 cells. The 54th Annual Meeting of the Society of Toxicology, 22-26 March, 2015, San Deigo, CA, USA.

 

• Belali OM, Ansari MA, Korashy HM. Sunitinib Induces Growth Inhibition and Apoptosis in Breast Cancer MDA-MB-231 Cells through FOXO3a Signaling Pathway. Experimental Biology conference, March 28-April 1, 2015, Boston, USA.

 

• Belali OM, Attafi IM, Murashid BA, Ansari MA, Korashy HM. Metformin Protects against 7, 12-Dimethylbenz[a]anthracene-Induced Breast Cancer initiation and Adduct Formation In Vivo Rat Model Though CYP1A1/CYP1B1 Pathway (Submitted).

 
 
 
 
 
 
 
 
 

Father’s Name:                      Mohammad Yunus Ansari
 
Date of Birth:                        1 September 1981
 
Marital Status:                       Married
 
Language Known:                Hindi, English and Urdu
 
Passport no:                           J5356700
 
Nationality:                            Indian
 
Permanent Address:             Mohd. Yunus Ansari
                                                Lohia Nagar Rajesultanpur
                                                Ambedkar Nagar, UP- 224175
                                                Ph: 09811417106
 
 
 
 
 

References available upon request"