Validation of meta Topolin in organogenesis, improved morpho-physio- chemical responses, and clonal fidelity analysis in Dioscorea pentaphylla L. - an underutilized yam species

Journal Article

Published in: https://www.sciencedirect.com/science/article/abs/pii/S0254629922000047

The present investigation describes the efficacy of aromatic and synthetic cytokinins, which influence the direct organogenesis, biochemical responses, and ex vitro and in vivo performance of the in vitro propagated plantlets of Dioscorea pentaphylla L., an underrated famine tuber crop. The axillary shoot bud induction was achieved on the Murashige and Skoog's (MS) medium supplemented with 1.0 mg L−1 meta Topolin (mT) (96.9%) and 1.0 mg L−1 Thidiazuron (TDZ) (82.4%). The sprouted shoots were proliferated using 0.5 mg L−1 mT combined with 0.1 mg L−1 indole-3-acetic acid (IAA) with the maximum average number of 43.0 ± 1.29 shoots (7.2 ± 0.55 cm length). The shoots proliferated on mT and IAA combination demonstrated the presence of higher concentrations of carbohydrates and photosynthetic pigment contents in the leaves as compared with TDZ and IAA derived leaves. The healthy micro-shoots were rooted on a half-strength MS medium containing 1.0 mg L−1 indole-3-butyric acid (IBA) with the development of 12.6 ± 0.38 roots (5.9 ± 0.22 cm length). The plantlets were acclimatized in a greenhouse and showed 100% survival under in vivo conditions. The clonal fidelity of the regenerants was evaluated for the first time using Start codon targeted (SCoT) markers. Thirteen primers amplified 62 well-resolved bands and ranged from 1-8 bands. There were no somaclonal variations detected among the regenerants of D. pentaphylla with the mother plant.

• Publisher Name: Elsevier
• Volume Number: 145
• Magazine \ Newspaper: South African Journal of Botany
• Pages: 284-292