Bioactivity of Syzygium aromaticum (L.) Merr. & L.M.Perry extracts as potential antimicrobial and anticancer agents
T., Yassin, M. . 2020
Objectives: Cancer is the most common cause of death every year. Moreover, high incidence of bacterial
resistance to the most frequently used antibiotics contributes a significant death and disability worldwide.
Hence, formulation of novel antimicrobial and anticarcinogenic agents is required.
Methods: In the current study, the antibacterial efficiency of clove extracts (acetonic, dichloromethane,
ethanolic, and petroleum ether) against four pathogenic bacterial strains [Escherichia coli, Salmonella
typhi, Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA)] was estimated by
disc diffusion method. Furthermore, the anticancer potency of S. aromaticum extracts against HCT human
colon carcinoma was appraised using MTT assay. The phytochemical ingredients of the most effective
extract were determined using gas chromatography–mass spectrometry (GC–MS) analysis.
Results and conclusion: The dichloromethane extract presented the highest antibacterial potency against
the pathogenic isolates recording minimum inhibitory concentration (MIC) of 1 mg/disc against MRSA
strain and 0.5 mg/disc against both of E. coli and S. typhi strains. Furthermore, the minimum bactericidal
concentration (MBC) of clove dichloromethane extract was recoded at 2 mg/disc against MRSA stain, and
1 mg/disc against E. coli and S. typhi strains. The dichloromethane extract of clove showed the lowest
cytotoxic activity against HCT cell line whereas, the ethanolic extract exerted the highest efficacy with
relative IC50 of 6.71 and 2.53 mg/ml respectively. GC–MS analysis revealed that the clove dichloromethane
extract was comprised of eugenol (50.65%) and eugenyl acetate (12.54%) as major active components. In
conclusion, clove extracts could be utilized as potential antibacterial and anticarcinogenic agents.
Groundwater-dependent vegetation (GDV) plays a vital role in maintaining biodiversity and ecosystem services in the Mediterranean biome but is increasingly threatened by climate and land use…
Plant functional traits are fundamental to ecosystem dynamics and Earth system processes, but their global characterization is limited by available field surveys and trait measurements.
Sabkhas, or salt marshes, are important wetland habitats that play a vital role in capturing and storing carbon. This research examines the capacity of six salt marshes to sequester…