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Dr. Javed Ahmad

Assistant Professor

Assistant Professor

كلية العلوم
Room # 2B 121, Second Floor, Bld # 5, Department of Zoology, College of Science
publication
Journal Article
2020

Anti-cancer efficacy of Aloe vera capped hematite nanoparticles in human breast cancer (MCF-7) cells

We have applied a green approach employing the Aloe vera bioactives to encapsulate the hematite nanoparticles (ALE-α-Fe2O3NPs), and invested its potential to kill human breast cancer cells (MCF-7). Cytotoxicity analysis by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] (MTT) and neutral red uptake (NRU) assays showed 36% and 33% reduction in cell viability at 100 μg/ml of ALE-α-Fe2O3NPs. Relative to untreated control, flow cytometric (FCM) analysis revealed 68.97% greater internalization of ALE-α-Fe2O3NPs in MCF-7 cells. FCM study further confirmed ALE-α-Fe2O3NPs induced reduction in mitochondrial membrane potential (ΔΨm), and increase in intracellular reactive oxygen species (ROS). MCF-7 cells treated with ALE-α-Fe2O3NPs demonstrated DNA damage quantified by the comet assay. MCF-7 cells explicitly showed an increase in sub-G1 peak (36.3%) at 100 μg/ml of ALE-α-Fe2O3NPs, confirming apoptotic cell death. qPCR data of apoptotic genes (p53, bax, caspase-3 and caspase-9) in MCF-7 cells showed enhanced upregulation upon ALE-α-Fe2O3NPs exposure. Overall, our data give a comprehensive, safe, cost-effective, and scalable biogenic nano-formulation of ALE-α-Fe2O3NPs for its putative clinical applications as anti-cancer drug.

Publication Work Type
Research
Publisher Name
Elsevier
Volume Number
60
Magazine \ Newspaper
Journal of Drug Delivery Science and Technology
Pages
102052
more of publication
publications

Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…

by Maqsood A. Siddiqui , Rizwan Wahab , Quaiser Saquib , Javed Ahmad , Nida N. Farshori , Ebtesam S. Al-Sheddi , Mai M. Al-Oqail , Shaza M. Al-Massarani , Abdulaziz A. Al-Khedhairy.
Published in:
ELZEVIER
publications

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…

2023
Published in:
ELZEVIER
publications

Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…

by Javed Ahmad a, Rizwan Wahab, Maqsood A. Siddiqui, Quaiser Saquib, Abdulaziz A. Al-Khedhairy.
2023
Published in:
ELZEVIER