BCH-530: Biochemical Methodology

v      Spectrometry: spectrophotometry (scanning), I.R. analysis, spectrofluorimetry.

v     Chromatography: purification of proteins, affinity and/or hydrophobic chromatography.

v     Electrophoresis: polyacrylamide gel electrophoresis (e.g. LDH    isoenzymes), SDS-polyacrylamide gel electrophoresis (molecular weight determination) Immunoelectrophoresis, isoelectric focusing.

v     Centrifugation: sub-cellular fractionation, molecular weight determination by sucrose density gradient.

v      Radioactive counting and radioimmunoassay

v      Gas chromatography of lipids

v      Transport of ions in biomembranes