Aspergillus sydowi Metabolites Efficacies against the Mosquito Larval (Culex pipiens and Aedes caspius) Population and Cytotoxicity after Purification with Column Chromatography. Research journal of biotechnology 9,(1), 84-89.
The present study was carried out to evaluate the
larvicidal activity and cytotoxicity of Aspergillus
sydowi extract. The LC50 calculated of methanol crude
extract and fraction 6 was 250.86±4.15 and
56.61±0.89 µg/ml for Aedes caspius and 271.02±7.01
and 103.74±0.99 for Culex pipina respectively after
24 hours of exposure. Larvae of Ae. caspius treated
with fraction 6 of A. sydowi methanol extract
underwent a lethal disruption of the peritrophic
membrane and an extensive cellular microvillar
disruption. Expression of Bcl-2 representative of
antiapoptotic protein was greatly reduced in control
when compared to treated group conversely; Bax the
pro-apoptotic protein was higher in treated group.
The cytotoxicity of fraction 6 and 7 showed 80.25%
and 86.3 % survival at the highest concentration
(37µg/ml) tested against Hepg2. On the other hand,
Jurkat cells lines showed higher sensitivity to the
fraction tested. Fraction 6 showed 45.17% survival
where as fraction 7 showed 86.34 % survival at the
highest concentration tested. The HPLC
chromatogram revealed that fraction 6 obtained is not
pure and requires further purification and
characterization to examine larvicidal and cytotoxic
effect.
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