Electroconductive bioplatform based on dextrin for the immobilization of hemoglobin: Application for electrochemical monitoring of H2O2.
A novel biodegradable dextrin-based nanocomposite, involving polypyrrole (PPy) and hydrophilic dextrin (Dex) (PPy@Dex) was prepared using in-situ radical chemical polymerization technique. The obtained PPy@Dexbionanocomposite was fully characterized by FT-IR, XRD, FESEM, and DSC methods. The exceptional properties such as biocompatibility, high surface area, the proper functional group on the surface, and outstanding electrical conductivity of synthesized bionanocomposite made it a superior candidate over biomolecules immobilization. Electrochemical observations revealed that the PPy@Dex-coated glassy carbon electrode (GCE) demonstrated improved performance, making it a suitable substrate for immobilizing hemoglobin (Hb) and constructing an efficient biosensor. The resulting biosensor, named Hb-PPy@Dex/GCE, exhibited high activity in the reduction of hydrogen peroxide (H2O2). Amperometric examinations demonstrated an extensive linear range from 2 to 350 μM for Hb-PPy@Dex/GCE. The detection limit of the proposed approach was calculated to be 0.54 μM, following the S/N =3 protocol.
As intervertebral discdegeneration(IVDD)proceeds, the dysfunctional mitochondria disrupt the viability of nucleus pulposuscells, initiatingthedegradationof theextracellularmatrix. To date, there…
The present study deals with two-phase non-Newtonian pseudo plastic crude oil and water flow inside horizontal pipes simulated by ANSYS. The study helps predict velocity and velocity profiles, as…
Developmental and technological activities result in uncontrollable release of synthetic dyes into water reservoirs posing potential threat to flora and fauna. Thus, the development of suitable…