Antimicrobial and cytotoxic activities of isoniazid connected menthone derivatives and their investigation of clinical pathogens causing infectious disease
https://doi.org/10.1016/j.jiph.2020.12.033
Background: This work is the development of new molecules of isoniazid derivatives as dealing with the potential
of antimicrobial activity against clinical pathogens causing infectious disease. Antimicrobial of novel
Mannich base derivatives can be achieved via the one-pot synthesis in the green chemistry approach. This method
offers efficient, mild reaction conditions and high yields. In this study, a totally 12 compounds (1a–l) was
prepared and screened for cytotoxic and antimicrobial activities.
Materials and methods: Newly synthesised compounds were conformed via FT- IR, 1H, and 13C NMR
(Nuclear Magnetic Resonance), and mass spectra analysis. All compounds were checked for antibacterial
activity against gram-positive bacteria of Enterococcus faecalis, Staphylococcus aureus and gram-negative
bacteria of Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. All compounds were
checked against antifungal activity against Aspergillus fumigatus, Candida albicans, Cryptococcus neofor-
mans, Aspergillus niger, and Microsporum audouinii. All compounds were screened for cytotoxic activity
against the MCF-7 (Michigan Cancer Foundation-7) cancer cell line.
Result: The compound 1g was highly (MIC: 0.25 g/mL) active against gram-negative bacterial of P.
aeruginosa, whereas other compounds 1e and 1h were more active (MIC: 2 g/mL) in K. pneumoniae and
also 1g (MIC: 2 g/mL) was more active in E. faecalis than standard ciprofloxacin. Antifungal screening,
the compound 1b was highly active (MIC: 0.25 g/mL) against C. albicance, 1g (MIC: 2 g/mL) and 1h
(MIC: 4 g/mL) was significant of active against A. fumigatus, and the compound 1c (MIC: 4 g/mL) was
extremely active in M. audouinii than clotrimazole. Compound 1g (GI50 = 0.01 M) exhibited high activity
against the MCF-7 cell line, while 1b (GI50 = 0.02 M) was equipotent active compared with standard
doxorubicin.
Conclusion: A novel set of isoniazid derivatives (1a–l) and 1h were synthesized and screened for antimi-
crobial and cytotoxic activities. We found some highly active molecules, which are evidencing to be a
potential treatment of bacterial and fungal infection candidates.
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