The Beneficial Effect of Eco-Friendly Green Nanoparticles Using Garcinia mangostana Peel Extract against Pathogenicity of Listeria monocytogenes in Female BALB/c Mic
5,6, Afrah F. Alkhuriji 1 , Nada A. Majrashi 1, Suliman Alomar 2, Manal F. El-Khadragy 1,3 , Manal A. Awad 4 , Alaa R. Khatab 1 and Hany M. Yehia . 2020
Abstract: Listeria monocytogenes is a psychrophilic bacterium, which causes widespread zoonosis in the natural environment, and mainly affects goat, sheep, and cattle herds. Recently, we predicted that it can be transmitted through food. It causes listeriosis, a severe infectious disease, which occurs with food contaminated with the pathogenic bacterium. Anti-inflammatory factors are important to treat the dangers of chronic inflammation associated with chronic diseases. Natural foodstuffs have made and are continuing to make vital contributions to the search for new antilisterial agents. The use of natural products in association with silver nanoparticles has drawn attention because of its easy, nonpathogenic, eco-friendly, and economical protocol. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using Garciniamangostana peel extract, which was found to be a good source forthesynthesisofsilvernanoparticles,theirformationbeingconfirmedbycolorchangeandstability in solution, and investigated the antilisterial activity of these nanoparticles in a murine model of L. monocytogenes infection. A total of 28 mice were divided into four groups—healthy control, infected, infected mice treated with green Ag-NPs biosynthesized with G. mangostana (5 mg/mL), and infected mice pretreated with Ag-NPs. From our results, oral treatment with Ag-NPs biosynthesized with G. mangostana peel extract resulted in a significant reduction in malondialdehyde (MDA), enhanced antioxidant enzyme activities, and increased the levels of the antiapoptotic protein, compared with the untreated mice. These results indicate that G.mangostana may provide therapeutic value against L.monocytogenes-inducedoxidativestressandhistopathologicalalterations,andthattheseeffectsmay be related to antiapoptotic and antioxidant activities.
Hepatitis C virus remained a public health problem with approximately half of the patients untreated and
undiagnosed. Chronic HCV is a leading cause of cirrhosis, fibrosis, hepatocellular…
Abstract: Methylenetetrahydrofolate reductase (MTHFR) deficiency is the most common genetic cause of
hyperhomocysteinemia, which has been implicated in the etiology of recurrent spontaneous…
study, the protective potential of chlorogenic acid (CGA), a caffeoylquinic acid isomer abundantly found in many
plants, was investigated against sodium arsenite (NaAsO2)-induced testicular…