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Ajamaluddin Malik

Associate Professor

Associate Professor

كلية العلوم
Building no 5, room no 2A56
المنشورات
مقال فى مجلة
2016

Structural and thermodynamic properties of kappa class glutathione transferase from Camelus dromedarius.

Ismael, Ajamaluddin Malik, Farid S. Ataya, Dalia Fouad, Nikolaos E. Labrou Abdulrahman M. Al-Senaidy, Mohamad A. . 2016

The Arabian camel, Camelus dromedarius is naturally adapted to extreme desert climate and has evolved protective mechanisms to limit oxidative stress. The mitochondrial kappa class glutathione transferase enzyme is a member of GST supergene family that represents an important enzyme group in cellular Phase II detoxification machinery and is involved in the protection against oxidative stress and xenobiotics. In the present study, C. dromedarius kappa class glutathione transferase (CdGSTK1-1) was cloned, expressed in E. coli BL21, purified and its structural, thermodynamic and unfolding pathway was investigated. The results showed that CdGSTK1-1 has unique trimeric structure, exhibits low thermostability and a complex equilibrium unfolding profile. It unfolds through three folding states with formation of thinly populated intermediate species. The melting points (Tm) of the first unfolding transition was 40.3±0.2°C and Tm of the second unfolding transition was 49.1±0.1°C. The van't Hoff enthalpy of the first and second transition were 298.7±13.2 and 616.5±2.4kJ/mol, respectively. Moreover, intrinsic fluorescence and near-UV CD studies indicates that substrate binding does not leads to major conformational changes in CdGSTK1-1.

رقم المجلد
88
مجلة/صحيفة
International Journal of Biological Macromolecules
الصفحات
313–319
مزيد من المنشورات
publications

Polyphosphate polymers are chains of phosphate monomers chemically
bonded together via phosphoanhydride bonds. They are found in all prokaryotic and
eukaryotic organisms and are among…

بواسطة Ajamaluddin Malik, Javed Masood Khan, Abdulaziz M. Al-Amri, Nojood Altwaijry, Prerna Sharma, Abdullah Alhomida, Priyankar Sen
publications

Phosphate additions in processed foods are a health risk that has been overlooked. This study examined the
effects of a permitted food additive (E452; Sodium Hexametaphosphate (SHMP)) on…

بواسطة Ajamaluddin Malik, Javed Masood Khan, Abdulaziz M. Al-Amri, Nojood Altwaijry, Abdullah S Alhomida, Mohammad Shamsul Ola
2023
publications

Protein aggregation is implicated in different human diseases. It also makes the protein less desirable candidate for industry as they exhibit reduced biological activity. Proteins tend to…

بواسطة Javed Masood Khan, Ajamaluddin Malik, Abdulaziz M. Al-Amri.
2023
تم النشر فى:
Journal of Molecular Liquids