Reserach Interests: Drug Metabolism is best thought of as an interdisciplinary field in which Synthetic Organic Chemistry, Analytical Chemistry, Spectroscopy, Biology, Enzymology, and Pharmacokinetics interact to promote better understanding of the biotransformation processes that affect Xenobiotics. My research interests include attempts to isolate, purify, identify, and characterize drug metabolites generated either by in-vitro metabolism models such as isolated liver hepatocytes and liver microsomes or by the in-vivo administration of drugs to small mammals or humans. Other objectives involve the identification of iso-enzymes involved in the metabolism of the drug(s) of interest and the investigation of unusual paths of metabolism both in in-vitro and in-vivo models. The process involves the extensive use of different analytical techniques such as Liquid Chromatography – Mass Spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy. The use of LC-MS, and in particular tandem or hybrid MS-MS techniques has become the mainstay for drug metabolism research and the area is expanding rapidly. LC-MS offers a fast, accurate and potentially superior alternative to traditional analytical techniques used for the ID and characterization of drugs and their metabolites, a factor that could contribute to the overall facilitation of the drug discovery process. Our laboratory is constantly being updated to cope with the rapid advances taking place in the field of drug metabolism research. The laboratory is equipped with a semi-preparative high performance liquid chromatography (HPLC) system for the isolation and purification of drug metabolites from a variety of biological matrices. Additionally, the laboratory has recently seen the addition of a triple quadrupole mass spectrometer (QQQ) coupled to a rapid resolution liquid chromatography front end. This valuable addition will facilitate the structure elucidation and characterization of drug metabolites, as well as the conductance of many routine and non-routine chromatographic analyses requiring MS detection for qualitative and/or quantitative studies. Funded Research: Dr. Kadi has recently (January '08) been awarded a SR ~1.3 million research grant from King Abdulaziz City for Science and Technology (KACST) to conduct a multi-faceted project involving synthetic medicinal chemistry, pharmacology and drug metabolism. Dr. Kadi is joined in the project by KSU Medicinal Chemistry professors Dr. Abdulrahman Al-Obaid and Dr. Hussein El-Subbagh as well as Pharmacology professor Dr. Othman AL-Shabanah. The project will provide the addition to Dr. Kadi's laboratory of a dedicated ion trap mass spectrometer for sturcutre elucidation of drug metabolites.