King Saud University
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تحميل الدليل التدريبي

أسئلة شائعة






Khaled M. Sharafeldin, Ph.D.

Associate Professor of Physiology

Applied Medical Sciences College,

Al-Majma'ah, King Saud University

Tel: 06-4311520

Ext.: 1457


 Research Interest

 Comet Assay

The figures show the images of damaged & normal  DNA that can be measured as what is called tail moment. This technique is famed of Comet assay or single cell gel electrophoresis (SCGE). The damage is represented by an increase of DNA fragments that have migrated out of the cell nucleus in the form of a characteristic streak similar to the tail of a comet. The DNA fragments are generated by DNA double strand breaks, single strand breaks and/or strand breaks induced by alkali-labile sites in the alkaline version of the assay. The length and fragment content of the tail is directly proportional to the amount of DNA damage

Comet assay is used in the last ten years in the field of tumors and examination the repairing DNA in cells. Currently, the application of this technique in the field of ecotoxicity is very interesting and promising. It includes the evaluation the damage of DNA, as tail moment and the rate of its repair and also the rate of cell appoptosis. Comet assay can be conducted in both in vitro and in vivo test systems and is increasingly being used in genotoxic testing of industrial chemicals, agrochemicals and pharmaceuticals. Comet assay is rapid (results in days), simple to perform, requires small amounts of test substance (25-50 mg) and can be performed in almost any eukaryotic cells (different animal organs). Comet assay serves as an important tool in the early drug development compounds as a mechanistic and genotoxic predictor.


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