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تحميل الدليل التدريبي

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CV of Dr. Amany A. Aly

Surname:                                 Aly

Forename:                               Amany

Middle name:                          Abdalla

Sex:                                         Female

Date of birth:                          9/12/1963

Nationality:                             Egyptian

Marital Status:                         Married

Number of children:                Two,a girl 19 and a boy16

Address:                                  -Egypt:21 Abdalla El-Kateb ,Dokki, Giza

                                                  Flat 2, Post cod. 12311.

                                                -Riyadh: Khaldia Towers-Khazzan street, Tower 3,

                                                 3027, Riyadh 11417, Kingdom of Saudi Arabia.

                                         

Telephone:                               00202-37491979 (home, Egypt),    

                                                 0020101347526(Mobile, Egypt)

                                                 0096614065050-ext:3043(Riyadh)

                                                 

EDUCATION:

 

 

 

1996 – 2001                            Ph. D. from the school of biochemistry and Molecular

                                                Biology Leeds University, UK.

                                                The thesis title ”Biophysical studies on phospholamban”.

 

          

                               

1994-1996                               work as a Lecturer assistance in Cairo University, Faculty  

                                                 of Science, Biophysics Department, Egypt

 

 

1990 – 1994                            M. Sc. In Biophysics (molecular)

Title of the thesis “Study of structural behaviour of biological molecules in model membrane system using Biophysical techniques” Cairo University, Faculty of Science, Biophysics

                                                Department, Egypt.

 

1988-1990                               Present in Amsterdam (Holland) accompanied my

                                                 husband during his study for PhD degree.

         

1986-1988                               work as a demonstrator in Cairo University, Faculty of

                                                 science, Biophysics Department, Egypt

                                                   

 

1985 – 1986                            Postgraduates courses (required for the degree of master of

                                                science).

 

 

1981 – 1985                            B. Sc. (Distinction with Class Honour) in Biophysics

Cairo University, Faculty of Science, Biophysics

                                                Department, Egypt

 

 

                                               

EMPLYMENT:

 

2004-2008                            King saud University

                                              Work as a professor assistance in King saud    

                                                University, Faculty of Science, Physics Department,

                                                Kingdom of Saudi Arabia. . Duties included,

                                                organizing work, teaching students and supervising

                                                postgraduate students. Examining a master thesis.

 

 

2001-2004                            Cairo University

                                              Work as a Lecturer in Cairo University, Faculty

                                               of science, Biophysics Department, Egypt. Duties included,

                                                organizing work, teaching students and researching work

   

 

1996-2001                             LEEDS university, UK

                                               Studying as a postgraduate student in school of                      

                                               biochemistry and molecular Biology for PhD degree,

                                               LEEDS University, UK.

 

 

1994-1996                               Cairo University

                                                Work as a Lecturer assistance in Cairo University, Faculty

                                                of science, Biophysics Department, Egypt. Duties included,

                                                 organizing work, teaching students and researching work

                                                                                            

1990-1994                               Cairo University

                                                Work as a demonstrator in Cairo University, Faculty of

                                                 science, Biophysics Department, Egypt. Duties included,

                                                 organizing work, teaching students and researching work                    

 

 

1988-1990                               Present in Amsterdam (Holland) accompanied my

                                                 husband during his study for PhD degree.

          

 

                                               

1985 – 1988                            Cairo University

                                                Work as a demonstrator in Cairo University, Faculty of

                                                 science, Biophysics Department, Egypt. Duties included,

                                                 organizing work, teaching students and researching work                    

 

 

 

SCIENTIFIC EXPERIENCE

 

M. Sc. In Biophysics (molecular)

Title of the thesis “Study of structural behaviour of biological molecules in model membrane system using Biophysical techniques” Cairo University, Faculty of Science, Biophysics Department, Egypt.

            In my M.Sc. thesis, I studied the effect of cholesterol percentage on the rigidity of the vesicles membrane as well as its effect in releasing the drugs from the encapsulated vesicles using light scattering technique.

 

PhD degree from UK, University of Leeds, School of Biochemistry and Molecular Biology, April 2001.

Title: Biophysical Studies on Phospholamban

            My PhD was about phospholamban (PLB) protein, the principal membrane substrate for cardiac protein kinases to control the calcium pump.

In my study, PLB has been expressed in Escherichia coli as a fusion protein with an N-terminal affinity purification tag (His6-thrombin cleavage site) immediately preceding the PLB protein. This was used to purify (tagged) PLB in significant amounts (3mg/L bacterial culture) in a single chromatographic step from a sample of SDS-solubilised inclusion body material. For ion channel studies the PLB protein was purified to homogeneity by reverse phase HPLC chromatography on C4 columns, which allowed recovery of 40% of protein loaded onto the column. The tagged PLB protein was reconstituted into vesicles and then incorporated into a black lipid bilayer, but this protein (taggedPLB) did not exhibit ion channel properties.

To investigate whether the affinity tag affected PLB function, the fusion protein was digested with thrombin and the PLB product was purified to homogeneity by reverse phase HPLC. This was then incorporated into black lipid membranes after reconstitution of PLB into vesicles, as before. In this format PLB displayed ion channel activity. Channel opening was rather erratic in character, producing current amplitudes of short duration but variable amplitude.  Standard electrophysiological analysis of this current traces was not possible, and so I developed a simple, logical methodology for the analysis of these data. The closed state of the PLB channel was readily identified in all experiments, and thus the absolute values (of current) that represent the closed state were deducted from all other current values in the data set (at that holding potential). An average conductance per unit time was then calculated for each data set, and used to construct average current/voltage relationships. In the presence of a ten-fold gradient of KCl, PLB displayed voltage dependent channel activity with mild anion selectivity.

To investigate the regulation of this ion channel I examined the effect of oligomeric status on channel function, and the effect of two important physiological regulators of PLB, namely Ser-16 phosphorylation and Ca2+ ions. A mutant form of PLB, C41A/C46A, was expressed in E. coli and purified to homogenity. This protein was monomeric in SDS-PAGE experiments, however it was able to form ion channels indistinguishable from wild type PLB when incorporated in black lipid membranes. From this I suspect that the oligomeric status of m5PLB in SDS does not reflect the oligomeric state of the protein in a lipid bilayer.

Wild type PLB was phosphorylated (on Ser-16) to stoichiometry using purified protein kinase A. Phosphorylated PLB exhibited voltage dependent ion channel activity with mild anion selectivity similar to the dephosphorylated channel. However the channel differed from dephosphorylated PLB in two respects: firstly, the channel activity was more orthodox, with channel openings generating current amplitude of more uniform size within each data sweep. Secondly, the average conductance of the phosphorylated channel was significantly greater than that of the dephosphorylated PLB.

Finally, I investigated the regulatory effects of Ca2+-ions. PLB and phosphorylated PLB channels were exposed to CaCl2 in the presence of the ten-fold gradient of KCl. In all cases it appeared that Ca2+, K+ and Cl- were permeant species, however in the case of the dephosphorylated PLB Ca2+ also altered the ion selectivity of the channel, causing it to become more permeable to cations.

 

Teaching experience

Teaching the following courses:

1-General biophysics for the student of  1st year faculty of science Cairo university (2001-2003).

2- General biophysics for the student of  2nd  year faculty of science Cairo university (2001-2003).

3-Radiation protection course for the postgraduate student and diploma of biophysics in Cairo university (2001-2004).

4- General biophysics for the student of final year faculty of science Cairo university (2001- 2004).

5- General biophysics for the master student in faculty of science Cairo University (2004).

6-General physics for the student of King Saud University (2004-2008).

7-General biophysics for the student of King Saud University (2004-2008).

8-wave phenomena for the student of King Saud University (2004-2008).

9-Elementry modern physics for the student of King Saud University (2004-2008).

PUBLICATIONS

1- Dielectric relaxation and light scattering studies for lipid/cholesterol liposomes

W. A. Khalil, A. Soltan Monem, M. Abutaleb, A. Abdalla and M. Ali Fadel

Egyptian J. of Biophys, Special Issue. “Molecular and Molecular Biophysics” (1994) 128

 

 

2- Studies of the induced effects of exposures to 0.1 mT 50 Hz magnetic fields on the biophysical properties of RBCs and ECG of rats (in vivo studies)

Reem El-Gebaly and Amany A. Aly

Biophysics department, Faculty of Science ,Cairo University, Egyptian  biophysical journal, December2003

 

 

3- Control of Ehrlich tumour growth by electromagnetic waves at resonance frequency (In vivo studies)

Fadel M. A., Reem H.El-Gebaly, Amany A. Aly and Fakhry F. Ibrahim*

 

Department of Biophysics , Faculty of Science ,Cairo University Egypt

 *  Department of Clinical pathology,Cairo University hospitals Egypt

Electromagnetic Biology And Medicine, volume24, number1, 2005

4-Effect of microwave radiation on some biophysical and cardiovascular properties of exposed rats. The 3rd meeting of sauid physical scientific society, 2-18 December 2006, in King saud university.  

Supervision

-Supervising a master research student, in king saud university.

 The Muster  title is  " Effect of Microwave Radiation on Some Biophysical and Cardiovascular Properties of the Exposed Rats" (2006-2007).

 

- Supervising a master research student, in king saud university.

 The Muster  title is "Radiation properties of some natural soil conditioners" (2007--)

 

 

 

 
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