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Guidelines_English_Final
تحميل الدليل التدريبي

أسئلة شائعة


Training Courses

- Morocco  (2008), Institute Agronomique et Veterinaire Hassan II, Agadir. Workshop on Real Time PCR for The identification of plant pathogenic bacteria.

- Denmark (2003) Training course on diagnosis of plant pathogenic bacteria by selective media, PCR, fatty acid analysis at Danish Agriculture Research Center.

- United Kingdom (2002).Central Science Laboratory “CSL”, York, UK.

The Training program was:

-  Media preparation.

-  Raising and use of reference cultures of Ralstonia solanacearum and other plant pathogenic bacteria.

-  Review of protocols for potato brown rot testing.

-  Isolation from diseased tomato plants and determination of causal agents (including Ralstonia, Xanthomonas, Erwinia).

-     Isolation of R. solanacearum from 200-tuber potato extracts on SMSA medium.

- Biovar medium preparation and testing of reference cultures and Egyptian isolates

-  Use of lateral flow test kits.

-  Detailed overview of CSL routine brown rot testing procedures (potatoes, water, weeds and potato processing waste).

-  Overview of general bacteriology and diagnostic procedures used at CSL.

-  Overview of methods for antibody production at CSL.

-  Introduction to conventional PCR and TaqMan real-time quantitative PCR.

-  Procedures for DNA extraction from potato extracts.

-  BOX-PCR on DNA extracts from reference and Egyptian R. solanacearum isolates.

-  Overview of R. solanacearum research at CSL.

-  Compilation of reports and discussions of all results.

- Netherlands (2002). Plant Protection Service Wageningen,Netherlands.

The Training program was:-

- Detection methods of pathogenic bacteria R. solanacearum (Brown Rot) and C. michiganensis subsp. sepedonicus (Ring Rot) by Polymerase Chain Reaction Assay (PCR), immunofluorescence test (IFT), Fluorescent in situ hybridization test (FISH ).

- Identification of the causal agents of brown rot and ring rot diseases in potato plants by  Agarose electrophoresis, REP-PCR test and fatty acid analysis, DNA isolation methods of R. solanacearum and C. michiganensis subsp. Sepedonicus.

-  Water and weed sampling and performing isolation tests on collected samples.

-  Laboratory Quality Management, methods for maintenance of bacteria.

- Diagnostic protocol and procedure on brown rot and ring rot accepted within the European Union.

-In Denmark (Augt, 2003). Danish Agriculture Research Center.

-     Training course on diagnosis of plant pathogenic bacteria C. michiganensis subsp. sepedonicus by selective media, PCR, fatty acid analysis.

-          Visiting for potato filed, packing and processing stations.

 

- Canada (2000). Center of Animal and Plant Health, Charlottetown, Canada.

The Training program was: 

           -Media preparation.

-Raising and use of reference cultures of C. michiganensis subsp. Sepedonicus.

-Detection methods of pathogenic bacteria C. michiganensis subsp. sepedonicus (Ring Rot) by ELISA and  immunofluorescence test (IFT).

-Review of protocols for potato ring rot testing.

-Isolation of C. michiganensis subsp. sepedonicus from 200-tuber potato extracts on selective     medium.

-Detailed overview of Center of Animal and Plant Health routine ring rot testing procedures (potatoes, water, weeds and potato processing waste).

-Overview of general bacteriology and diagnostic procedures used at Center of Animal and Plant Health.

-Overview of methods for antibody production at Center of Animal and Plant Health.

 

Canada (2000).

-  Member of plant quarantine committee to inspection potato tubers against bacterial diseases.

 

Pakistan (2003).

-  Member of plant quarantine committee to inspection wheat plants against bacterial diseases.

 

 

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